Induction of the Sulfolobus shibatae virus SSV1 DNA replication by mitomycin C  

作　　者：Danxu Liu Li Huang 

机构地区：[1]Chinese Acad Sci, State Key Lab Microbial Resources, Inst Microbiol, Beijing 100080, Peoples R China

出　　处：《Chinese Science Bulletin》2002年第11期923-927,共5页

基　　金：This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770009 and 39925001);the fund for Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-3-01-02).

摘　　要：The temperate virus SSV1 from the hyper-thermophilic archaeon Sulfolobus shibatae provides a useful model system for the study of archaeal DNA replication. Southern hybridization showed that SSV1 existed primarily as a provirus in its host that was grown without shaking. Upon UV or mitomycin C induction, the cellular level of free SSV1 DNA increased drastically whereas that of integrated viral DNA remained unchanged. The results of mitomycin C induction were more reproducible than those of UV induction. We found that, when the cells that had been grown without shaking were shaken, the replication of SSV1 DNA was also induced. Based on our results, we developed a method for the induction of SSY1 DNA replication by mitomycin C. When the S. shibatae virus production was induced using this method, the cellular level of free SSV1 DNA started to increase 10 h after induction, and peaked after 12-15 h. A fully induced S. shibatae cell contained -50 molecules of free SSV1 DNA. The development of this inductionThe temperate virus SSV1 from the hyperthermophilic archaeon Sulfolobus shibatae provides a useful model system for the study of archaeal DNA replication. Southern hybridization showed that SSV1 existed primarily as a provirus in its host that was grown without shaking. Upon UV or mitomycin C induction, the cellular level of free SSV1 DNA increased drastically whereas that of integrated viral DNA remained unchanged. The results of mitomycin C induction were more reproducible than those of UV induction. We found that, when the cells that had been grown without shaking were shaken, the replication of SSV1 DNA was also induced. Based on our results, we developed a method for the induction of SSV1 DNA replication by mitomycin C. When the S. shibatae virus production was induced using this method, the cellular level of free SSV1 DNA started to increase 10 h after induction, and peaked after 12–15 h. A fully induced S. shibatae cell contained ～ 50 molecules of free SSV1 DNA. The development of this induction method and the description of the process of SSV1 DNA replication following induction are valuable to the analysis of the origin and mode of replication of the virus.

关 键 词：HYPERTHERMOPHILIC ARCHAEA SULFOLOBUS shibatae SSV1 DNA REPLICATION induction. 

分 类 号：S432.41[农业科学—植物病理学]