supported by a research grant from the Natural Sciences and Engineering Research Council of Canada(NSERC)to K.M.
Hyperthermus butylicus is a hyperthermophilic crenarchaeon that produces 1-butanol as an end product.A thermostable alcohol dehydrogenase(ADH)must be present in H.butylicus to act as the key enzyme responsible for thi...
the National Natural Science Foundation of China (41525011, 91751205, and 31661143022);the Deep Carbon Observatory project.
The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain larg...
Supported by the National Natural Science Foundation of China(Nos.30870539, 21072075);the 38th Postdoctoral Foundation of China(No.801050321413)
The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547(Aeropyrum pernix esterase 1547). APE 1547 used in this reaction showed a remarkable stereodi...
supported by the National Basic Research Program of China(973 Program);the National Natural Science Foundation of China.
The inherent evolvability of promiscuous enzymes endows them with great potential to be artificially evolved for novel functions.Previously,we succeeded in transforming a promiscuous acylaminoacyl peptidase(apAAP)from...
Supported by the National Basic Research Program of China (Grant No. 2004CB719604);National Natural Science Foundation of China (Grant Nos. 30470386 and 30700011)
ST0838 (designed stRad55B) is one of the four RadA paralogs (or Rad55 homologues) in the genome of the hyperthermophilic crenarchaeon Sulfolobus tokodaii. The gene is induced by UV irradiation, sug-gesting that it is ...
Supported by the National Natural Science Foundation of China(Nos30400081, 30570405 and 20672045);the Key Tech-nology Research and Development Program of China(No2004BA713D03-04)
To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of (R, S)-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a ...
This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770009 and 39925001);the fund for Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-3-01-02).
The temperate virus SSV1 from the hyper-thermophilic archaeon Sulfolobus shibatae provides a useful model system for the study of archaeal DNA replication. Southern hybridization showed that SSV1 existed primarily as ...
This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770006 & 39925001)
The thermoacidophilic archaeon Sulfolobus shibatae synthesizes a large amount of the 7-ku DMA binding proteins known as Ssh7. Our hybridization experiments showed that two Ssh7-encoding genes existed in the genome of ...
Project supported by the National Natural Science Foundation of China (Grant No. 39770006);the Irvine Foundation and the Director's fund in the Institute of Microbiology;the Chinese Academy of Sciences, and a Grant-in-Aid for Scientists Returning f
An 11.5-ku DNA binding protein, designated as Sshl2, was purified from the hyperthermophilic archaeon Sulfolobus shibatae by column chromatography in SP Sepharose, DNA cellulose and phosphocellulose. Sshl2 accounts fo...