HYPERTHERMOPHILIC

作品数:10被引量:9H指数:1
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相关领域:生物学更多>>
相关机构:东南大学更多>>
相关期刊:《Science China(Life Sciences)》《mLife》《Chemical Research in Chinese Universities》《Science Bulletin》更多>>
相关基金:国家自然科学基金国家重点基础研究发展计划中国博士后科学基金更多>>
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A novel alcohol dehydrogenase in the hyperthermophilic crenarchaeon Hyperthermus butylicus
《mLife》2024年第2期317-325,共9页Ching Tse Kesen Ma 
supported by a research grant from the Natural Sciences and Engineering Research Council of Canada(NSERC)to K.M.
Hyperthermus butylicus is a hyperthermophilic crenarchaeon that produces 1-butanol as an end product.A thermostable alcohol dehydrogenase(ADH)must be present in H.butylicus to act as the key enzyme responsible for thi...
关键词:BUTANOL HYPERTHERMOPHILE Hyperthermus butylicus novel alcohol dehydrogenase thermostability 
Core Metabolic Features and Hot Origin of Bathyarchaeota被引量:6
《Engineering》2019年第3期498-504,共7页Xiaoyuan Feng Yinzhao Wang Rahul Zubin Fengping Wang 
the National Natural Science Foundation of China (41525011, 91751205, and 31661143022);the Deep Carbon Observatory project.
The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain larg...
关键词:Bathyarchaeota METAGENOMICS COMPARATIVE GENOMICS HYPERTHERMOPHILIC adaptation 
Molecular Basis for Stereospecific Hydrolysis of Ethyl Mandelate by Thermophilic Esterase
《Chemical Research in Chinese Universities》2011年第5期841-844,共4页ZHANG Guo-yan TAO Jin ZHENG Liang-yu CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos.30870539, 21072075);the 38th Postdoctoral Foundation of China(No.801050321413)
The stereospecific hydrolysis of mandelate can be effectively catalyzed by hyperthermophilic acylpeptide esterase APE 1547(Aeropyrum pernix esterase 1547). APE 1547 used in this reaction showed a remarkable stereodi...
关键词:Hyperthermophilic acylpeptide esterase BIOCATALYST Stereospecific hydrolysis Mandelic acid Computer simulation 
Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering
《Protein & Cell》2011年第6期497-506,共10页Chang Liu Guangyu Yang Lie Wu Guohe Tian Zuoming Zhang Yan Feng 
supported by the National Basic Research Program of China(973 Program);the National Natural Science Foundation of China.
The inherent evolvability of promiscuous enzymes endows them with great potential to be artificially evolved for novel functions.Previously,we succeeded in transforming a promiscuous acylaminoacyl peptidase(apAAP)from...
关键词:acylaminoacyl peptidase ESTERASE substrate specificity protein engineering solvent engineering 
Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaii
《Science China(Life Sciences)》2008年第1期60-65,共6页SHENG DuoHong, LI MingFeng, JIAO JianDong, NI JinFeng & SHEN YuLong State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China 
Supported by the National Basic Research Program of China (Grant No. 2004CB719604);National Natural Science Foundation of China (Grant Nos. 30470386 and 30700011)
ST0838 (designed stRad55B) is one of the four RadA paralogs (or Rad55 homologues) in the genome of the hyperthermophilic crenarchaeon Sulfolobus tokodaii. The gene is induced by UV irradiation, sug-gesting that it is ...
关键词:ARCHAEA recombinational repair RADA Rad55 CO-EXPRESSION 
High-throughput Screening of the Enantioselectivity of Hyperthermophilic Mutant Esterases from Archaeon Aeropyrum pernix K1 for Resolution of (R,S)-2-Octanol Acetate被引量:1
《Chemical Research in Chinese Universities》2007年第3期319-324,共6页ZHANG Gui-rong GAO Ren-jun ZHANG Ai-jun RAO Lang CAO Shu-gui 
Supported by the National Natural Science Foundation of China(Nos30400081, 30570405 and 20672045);the Key Tech-nology Research and Development Program of China(No2004BA713D03-04)
To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of (R, S)-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a ...
关键词:High-throughput screening ENANTIOSELECTIVITY Hyperthermophilic esterase Directed evolution 
Biobleach boosting effect of recombinant xylanase B from the hyperthermophilic Thermotoga maritima on wheat straw pulp被引量:1
《中国生物学文摘》2007年第3期13-13,共1页Jiang, Z. Q. Li, X. T. Yang, S. Q. Li, L. T. Li, Y. Feng, W. Y. 
关键词:麦杆浆粕 牛皮纸浆 木聚糖酶 热稳定性 漂白 造纸工业 
Induction of the Sulfolobus shibatae virus SSV1 DNA replication by mitomycin C
《Chinese Science Bulletin》2002年第11期923-927,共5页Danxu Liu Li Huang 
This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770009 and 39925001);the fund for Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KSCX2-3-01-02).
The temperate virus SSV1 from the hyper-thermophilic archaeon Sulfolobus shibatae provides a useful model system for the study of archaeal DNA replication. Southern hybridization showed that SSV1 existed primarily as ...
关键词:HYPERTHERMOPHILIC ARCHAEA SULFOLOBUS shibatae SSV1 DNA REPLICATION induction. 
Evolutionary conservation and DNA binding properties of the Ssh7 proteins from Sulfolobus shibatae
《Science China(Life Sciences)》2002年第6期583-592,共10页陈绪林 郭荣 黄力 Ray Hong 
This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770006 & 39925001)
The thermoacidophilic archaeon Sulfolobus shibatae synthesizes a large amount of the 7-ku DMA binding proteins known as Ssh7. Our hybridization experiments showed that two Ssh7-encoding genes existed in the genome of ...
关键词:HYPERTHERMOPHILIC archaea  DNA BINDING proteins  ssh7a/ssh7b genes  PROTEIN-DNA interaction. 
Effect of DNA binding protein Ssh12 from hyperthermophilic archaeon Sulfolobus shibatae on DNA supercoiling被引量:1
《Science China(Life Sciences)》1999年第4期401-408,共8页楼慧强 黄力 VietQ.Mai 
Project supported by the National Natural Science Foundation of China (Grant No. 39770006);the Irvine Foundation and the Director's fund in the Institute of Microbiology;the Chinese Academy of Sciences, and a Grant-in-Aid for Scientists Returning f
An 11.5-ku DNA binding protein, designated as Sshl2, was purified from the hyperthermophilic archaeon Sulfolobus shibatae by column chromatography in SP Sepharose, DNA cellulose and phosphocellulose. Sshl2 accounts fo...
关键词:HYPERTHERMOPHILIC ARCHAEA SULFOLOBUS shibatae DNA BINDING PROTEIN supercoiling. 
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