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Single-cell protein-DNA interactomics and multiomics tools for deciphering genome regulation: 《National Science Open》2023年第3期64-82,共19页Haiqing Xiong Runyu Wang Aibin He; supported by the National Key Research and Development Program of China(2021YFA1100101 and 2017YFA0103402);the National Natural Science Foundation of China(32025015 and 31771607);the Peking-Tsinghua Center for Life Sciences.; The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies,providing an unprecedented opportunity for decoding cell fate decisions largely enc...; 关键词：single-cell protein-DNA interaction single-cell multiomics single-cell epigenomics epigenome genome regulation

Step-growth polymerization of traptavidin-DNA conjugates for plasmonic nanostructures: 《Chinese Chemical Letters》2020年第5期1137-1140,共4页Young-Youb Kim Yongbin Bang Dayoung Lee Mingyu Kang Yoon-Kyu Song; supported by the Brain Research Program(No.2016M3C7A1904987)through the National Research Foundation of Korea(NRF)。; Here,we use two important biomaterials,protein and DNA,to construct self-assembled linear nanostructures through Watson-Crick base-paring of DNAs.We apply a simple magnetic separation method to purify traptavidin-DNA ...; 关键词：Protein-DNA conjugate DNA nanostructure Supramolecular assembly Traptavidin Plasmonic nanostructure

Crystal structure of Cas1 in complex with branched DNA被引量：2: 《Science China(Life Sciences)》2020年第4期516-528,共13页Jing Yang Jiazhi Li Jiuyu Wang Gang Sheng Min Wang Hongtu Zhao Yanhua Yang Yanli Wang; The research was funded by the National Natural Science Foundation of China(31630015,31725008);the Chinese Academy of Sciences(QYZDY-SSW-SMC021).; Cas1 is a key component of the CRISPR adaptation complex,which captures and integrates foreign DNA into the CRISPR array,resulting in the generation of new spacers.We have determined crystal structures of Thermus ther...; 关键词：CRISPR CAS protein Cas1 ADAPTATION DNA integration structure PROTEIN-DNA COMPLEX

The applications of nanopores in studies of proteins被引量：2: 《Science Bulletin》2019年第19期1456-1467,共12页Taoli Ding Antony KChen Zuhong Lu; supported by the National Key R&D Program of China(2016YFA0501602);a grant from the Beijing Science and Technology Commission(Z151100003915081); Nanopores are a label-free platform with the ability to detect subtle changes in the activities of individual biomolecules under physiological conditions.Here,we comprehensively review the technological development of...; 关键词：NANOPORES PROTEINS PROTEIN-PROTEIN INTERACTIONS PROTEIN-DNA INTERACTIONS

3eciphering the protein-DNA code of bacteria Ninged helix-turn-helix transcription factors: 《Frontiers of Electrical and Electronic Engineering in China》2018年第1期68-84,共17页Adam P. Joyce James J. Havranek; Background： Sequence-specific binding by transcription factors （TFs） plays a significant role in the selection and regulation of target genes. At the protein：DNA interface, amino acid side-chains construct a diver...; 关键词：transcription factor SELEX winged helix-turn-helix specificity detenlainants two-component signaling

Modeling the specificity of protein-DNA nteractions被引量：4: 《Frontiers of Electrical and Electronic Engineering in China》2013年第2期115-130,共16页Gary D. Stormo; The specificity of protein-DNA interactions is most commonly modeled using position weight matrices （PWMs）. First introduced in 1982, they have been adapted to many new types of data and many different approaches ha...

Interaction of zinc finger ZNF191(243—368) and its I323W and R327W mutants with oligonucleotides by florescence spectrum: 《Science China Chemistry》2003年第1期64-74,共11页周晶刘玉奇汤李明滕昕辰唐宁黄仲贤; This work was supported by the National Natural Science Foundation of China (Grant Nos. 30170228 and 39990600).; Two mutants of the zinc finger protein, ZNF191(243—368) I323W and R327W, are suc-cessfully obtained by site-directed mutagenesis. The fluorescence spectrum is used to study the interaction between these two mutants a...; 关键词：zinc finger protein ZNF191(243-368) SITE-DIRECTED mutagenesis fluorescence spectrum PROTEIN-DNA interaction.

Evolutionary conservation and DNA binding properties of the Ssh7 proteins from Sulfolobus shibatae: 《Science China(Life Sciences)》2002年第6期583-592,共10页陈绪林郭荣黄力 Ray Hong; This work was supported by the National Natural Science Foundation of China (Grant Nos. 39770006 & 39925001); The thermoacidophilic archaeon Sulfolobus shibatae synthesizes a large amount of the 7-ku DMA binding proteins known as Ssh7. Our hybridization experiments showed that two Ssh7-encoding genes existed in the genome of ...; 关键词：HYPERTHERMOPHILIC archaea DNA BINDING proteins ssh7a/ssh7b genes PROTEIN-DNA interaction.

Effect of DNA methylation on protein-DNA interaction of HL-60 cells: 《Science China(Life Sciences)》1999年第5期501-505,共5页何忠效白坚石张昱; HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as in-ducer in the presence of optimum concentration of 10 μmol/L. The methylation level of genome DNA determined by HPLC ...; 关键词：S-ADENOSYL-L-METHIONINE (SAM) HL-60 cell DNA METHYLATION PROTEIN-DNA interaction.

A　COMPLEX　OF　INTERMEDIATE　FILAMENT　PROTEIN－DNA：A　TARGET　FOR　AUTOANTIBODIES　IN　SYSTEMIC　LUPUS　ERYTHEMATOSUS？: 《Chinese Medical Sciences Journal》1995年第2期90-92,共3页阎锡蕴; Autoantibodies in systemic lupus erythematosus which cross-react with double stranded DNA and intermediate filament proteins are frequently reported. However, little is Known about the origin and the target of these a...; 关键词：monoclonal antibody lupus erythematosus AUTOANTIBODY

PROTEIN-DNA