Effect of DNA methylation on protein-DNA interaction of HL-60 cells  

作　　者：何忠效 白坚石 张昱 

出　　处：《Science China(Life Sciences)》1999年第5期501-505,共5页中国科学（生命科学英文版）

摘　　要：HL-60 cells have been induced with differentiation index 16% by S-adenosyl-L-methionine (SAM) as in-ducer in the presence of optimum concentration of 10 μmol/L. The methylation level of genome DNA determined by HPLC is increased during cell differentiation. When restriction endonuclease Hae Ⅲ, Sma Ⅰ, Sal Ⅰ, Xho Ⅰ and Hind III which are sensitive to 5-methylcytosirte were used to cleave the genome DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.HL-60 cells have been induced with differentiation index 16 % by S-adenosyl-L-rnethionine (SAM) as inducer in the presence of optimum conceptration of 10 μmol/L. The methylation level of genorne DNA determined by HPLC is increased during cell differentiation. When restriction endonuclease Hae Ⅲ, Sma I, Sal I, XhoI and Hind Ⅲ which are sensitive to 5-methylcytosine were used to cleave the genorne DNA, a resistance effect was found. The interaction between DNA and DNA binding proteins is changed by using gel retarding test.

关 键 词：S-ADENOSYL-L-METHIONINE (SAM) HL-60 cell DNA METHYLATION PROTEIN-DNA interaction. 

分 类 号：Q52[生物学—生物化学]