Secretion of Plasminogen Activator Inhibitor Type 1 by Cultured Ovarian Cells Obtained From Gonadotropin-treated Immature Rats  

作　　者：刘以训 冯强 彭晓蓉 Tor Ny 

机构地区：[1]Laboratory of Reproductive Biology,Institute of Zoology,Academia Sinica,Beijing 100080,PRC [2]Department of Applied Cell and Molecular Biology,University of Ume,S-90187,Ume,Sweden

出　　处：《Science China Chemistry》1994年第8期940-947,共8页中国科学（化学英文版）

基　　金：the Swedish Medical Research Council; the National Natural Science Foundation of China.

摘　　要：It is demonstrated that i) theca-interstitial compartment synthesizes the majority ofplasminogen activator inhibitor type 1 (PAI-1) in the ovary before ovulation,and the follicular wall maytherefore serve as a specific barrier with the presence of PAI-1 activity to prevent the secretion of tPA intothe extrafollicular compartments;ii) granulosa cells secrete only a small amount of ovarian PAI-1,butsynthesize the most of tissue-type plasminogen activator tPA involved in the processes leading to ovula-tion;iii) since only matured cumulus-oocyte complexes secrete a large amount of tPA and PAI-1,bothtPA and PAI-1 activity in the conditioned medium may be used as reliable markers for evaluating oocytequality for in vitro fertilization.It is demonstrated that i) theca-interstitial compartment synthesizes the majority of plasminogen activator inhibitor type 1 (PAI-1) in the ovary before ovulation,and the follicular wall may therefore serve as a specific barrier with the presence of PAI-1 activity to prevent the secretion of tPA into the extrafollicular compartments;ii) granulosa cells secrete only a small amount of ovarian PAI-1,but synthesize the most of tissue-type plasminogen activator tPA involved in the processes leading to ovula- tion;iii) since only matured cumulus-oocyte complexes secrete a large amount of tPA and PAI-1,both tPA and PAI-1 activity in the conditioned medium may be used as reliable markers for evaluating oocyte quality for in vitro fertilization.

关 键 词：PLASMINOGEN activator inhibitor TYPE 1 rat GRANULOSA CELLS theca-interstltial CELLS CUMULUS CELLS 

分 类 号：R96[医药卫生—药理学]