Treatment of β-Thalassemia With Hydroxyurea (HU)——Effects of HU on Globin Gene Expression  

作　　者：黄淑帧 任兆瑞 陈美珏 许洪平 曾溢滔 G.P.Rodgers 曾凡一 A.N.Schechter 

机构地区：[1]Shanghai Institute of Medical Genetics,Shanghai Children's Hospital,Shanghai 200040,PRC [2]Laboratory ofChemical Biology,National Institute of Diabetes,Digestive and Kidney Diseases,NIH,MD 20892,USA

出　　处：《Science China Chemistry》1994年第11期1350-1359,共10页中国科学（化学英文版）

基　　金：the Chinese"863"Research Grant and NIH Grant HLB-29623

摘　　要：A newly developed method of RT-PCR/competitive PCR for measuring the relative and ab-solute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study thealterations of globin gene expressions in the patients with β-thalassemia pre-and post-hydroxyurea(HU)treatment.It was found for the first time that HU had the effect of enhancing β-globin gene expression insome patients.Two cases with β-thalassemia who were subjected to HU treatment for over two years showeda marked increase in β-globin mRNA level and β-globin chain synthesis,resulting in more effective erythro-poiesis and the alleviation of clinical symptoms.A newly developed method of RT-PCR/competitive PCR for measuring the relative and ab- solute content of globin mRNAs as well as micro-globin chain biosynthetic assay have been used to study the alterations of globin gene expressions in the patients with β-thalassemia pre-and post-hydroxyurea(HU) treatment.It was found for the first time that HU had the effect of enhancing β-globin gene expression in some patients.Two cases with β-thalassemia who were subjected to HU treatment for over two years showed a marked increase in β-globin mRNA level and β-globin chain synthesis,resulting in more effective erythro- poiesis and the alleviation of clinical symptoms.

关 键 词：Β-THALASSEMIA GLOBIN GENE EXPRESSION HYDROXYUREA 

分 类 号：Q78[生物学—分子生物学]