A study on liver mitochondria respiration and protein synthesis in cold adapted rats  

作　　者：何开跃 杨泽田 

机构地区：[1]Central Laboratory,Fourth Military Medical University,Xi'an 710032

出　　处：《Journal of Medical Colleges of PLA(China)》1992年第1期45-48,共4页中国人民解放军军医大学学报（英文版）

摘　　要：Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func-tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio(RCR),was determined by oxygen electrode method,The protein synthesis in mitochondria wasstudied by observing the incorporation of[~3H]-Leucine into mitochondria.Polyacrylamide gelelectrophoresis was carried out to detect the changes of the inner membrane proteins.It was shownthat the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and returned tothe control level in the 6th week,the protein synthesis of mitochondria decreased significantly incold adapted rats in 1,2 and 4 weeks;the electrophoretic pattern of the inner membrane proteinsin mitochondria from cold adapted rat livers revealed some new bands.Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func- tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio (RCR),was determined by oxygen electrode method,The protein synthesis in mitochondria was studied by observing the incorporation of[~3H]-Leucine into mitochondria.Polyacrylamide gel electrophoresis was carried out to detect the changes of the inner membrane proteins.It was shown that the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and returned to the control level in the 6th week,the protein synthesis of mitochondria decreased significantly in cold adapted rats in 1,2 and 4 weeks;the electrophoretic pattern of the inner membrane proteins in mitochondria from cold adapted rat livers revealed some new bands.

关 键 词：COLD adaptation MITOCHONDRIA liver oxidative PHOSPHORYLATION RESPIRATORY control ratio protein synthesis membrane proteins RATS 

分 类 号：R[医药卫生]