Tumor localization of anti-gastric cancer monoclonal antibody MGb_2-daunomycin conjugate in nude mice bearing human gastric carcinoma SGC-7901  

作　　者：陈敏 黎松 邓敬兰 张学庸 陈希陶 

机构地区：[1]Department of Nuclear Medicine Xijing Hospital,Fourth Military Medical University,Xi'an 710032 [2]Laboratory of Gastroenterology,Xijing Hospital,Fourth Military Medical University,Xi'an 710032

出　　处：《Journal of Medical Colleges of PLA(China)》1992年第1期81-83,共3页中国人民解放军军医大学学报（英文版）

摘　　要：In the present study,an anti-gastric cancer monoclonal antibody MGb₂-daunomycin（DM）conjugate was prepared by cis-aconitic anhydride method.The distribution of the conjugatein nude mice bearing human gastric carcinoma SGC-7901 was investigated.Four to five moleculesof DM were introduced into each molecule of antibody while the antigen-binding capacity ofthe antibody was well-retained.This conjugate could be satisfactorily localized in the tumortissue.Ninety-six hours after intraperitoneal injection of ¹²⁵I-MGb₂-DM conjugate,the ratio ofradioactivity per milligram of tumor to that per milligram of blood reached 203,the result beingquite similar to that of unmodified MGb₂.In the present study,an anti-gastric cancer monoclonal antibody MGb_2-daunomycin (DM)conjugate was prepared by cis-aconitic anhydride method.The distribution of the conjugate in nude mice bearing human gastric carcinoma SGC-7901 was investigated.Four to five molecules of DM were introduced into each molecule of antibody while the antigen-binding capacity of the antibody was well-retained.This conjugate could be satisfactorily localized in the tumor tissue.Ninety-six hours after intraperitoneal injection of ^(125)I-MGb_2-DM conjugate,the ratio of radioactivity per milligram of tumor to that per milligram of blood reached 203,the result being quite similar to that of unmodified MGb_2.

关 键 词：stomach neoplasms antibodies monoclonal deunomycin antibody-toxin CONJUGATES iodine radioisotops mice NUDE 

分 类 号：R[医药卫生]