Corticotropin-releasing factor receptor subtype 2 in human colonic mucosa: Down-regulation in ulcerative colitis  

作　　者：Ekaterini Chatzaki Peter A Anton Mulugeta Million Maria Lambropoulou Theodoros Constantinidis George Kolios Yvette Taché Dimitri E Grigoriadis 

机构地区：[1]Neurocrine Biosciences Inc., San Diego, CA 92121, United States [2]Laboratory of Pharmacology, Faculty of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece [3]Center for HIV Prevention Research, Division of Digestive Diseases, University of California at Los Angeles, and VA Greater Los Angeles Healthcare System, Los Angeles, CA 90073, United States [4]Digestive Diseases Research Center and Center for Neurobiology of Stress, Division of Digestive Diseases, University of California at Los Angeles, and VA Greater Los Angeles Healthcare System, Los Angeles, CA 90073, United States [5]Laboratory of Histology-Embryology, Faculty of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece [6]Laboratory of Hygiene and Environmental Protection, Faculty of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece

出　　处：《World Journal of Gastroenterology》2013年第9期1416-1423,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Institute of Diabetes and Digestive and Kidney Diseases R01 grant DK-57238;Center Grant DK-41301 (Clinical core);Veteran Administration Research Career Scientist Award (YT);NIH DK-78676 (MM)

摘　　要：AIM: To assess corticotropin-releasing factor receptor 2 (CRF 2 ) expression in the colon of healthy subjects and patients with ulcerative colitis (UC). METHODS: We examined CRF2 gene and protein expression in the distal/sigmoid colonic mucosal biopsies from healthy subjects and patients with UC (active or disease in remission), human immunodeficiency virus (HIV) and functional bowel disease (FBD) by reverse transcriptionpolymerase chain reaction and immunofluorescence. RESULTS: Gene expression of CRF2 was demonstrated in the normal human colonic biopsies, but not in the human colorectal adenocarcinoma cell line Caco2. Receptor protein localization showed immunoreactive CRF 2 receptors in the lamina propria and in the epithelial cells of the distal/sigmoid biopsy samples. Interestingly, CRF 2 immunoreactivity was no longer observed in epithelial cells of patients with mild-moderately active UC and disease in remission, while receptor protein expression did not change in the lamina propria. No differences in CRF 2 expression profile were observed in distal/sigmoid intestinal biopsies from HIV infection and FBD patients, showing no signs of inflammation. CONCLUSION: The down-regulation of the CRF2 receptor in the distal/sigmoid biopsies of UC patients is indicative of change in CRF 2 signalling associated with the process of inflammation.AIM: To assess corticotropin-releasing factor receptor 2 (CRF2) expression in the colon of healthy subjects and patients with ulcerative colitis (UC). METHODS: We examined CRF2 gene and protein expression in the distal/sigmoid colonic mucosal biopsies from healthy subjects and patients with UC (active or disease in remission), human immunodeficiency virus (HIV) and functional bowel disease (FBD) by reverse transcription-polymerase chain reaction and immunofluorescence. RESULTS: Gene expression of CRF2 was demonstrated in the normal human colonic biopsies, but not in the human colorectal adenocarcinoma cell line Caco2. Receptor protein localization showed immunoreactive CRF2 receptors in the lamina propria and in the epithelial cells of the distal/sigmoid biopsy samples. Interestingly, CRF2 immunoreactivity was no longer observed in epithelial cells of patients with mild-moderately active UC and disease in remission, while receptor protein expression did not change in the lamina propria. No differences in CRF2 expression profile were observed in distal/sigmoid intestinal biopsies from HIV infection and FBD patients, showing no signs of inflammation. CONCLUSION: The down-regulation of the CRF2 receptor in the distal/sigmoid biopsies of UC patients is indicative of change in CRF2 signalling associated with the process of inflammation.

关 键 词：Colonic mucosa Corticotropin-releasing FACTOR Corticotropin-releasing FACTOR receptor HUMAN immunodeficiency virus ULCERATIVE colitis UROCORTIN 

分 类 号：R574.62[医药卫生—消化系统]