酵母胞内核黄素含量测定方法的研究  

Study on Determining Methods of Intracellular Riboflavin of Yeast

作　　者：李长文[1] 肖冬光[1] .韩英素[1] 李志勇[1] 

机构地区：[1]天津科技大学食品科学与生物工程学院

出　　处：《天津师范大学学报（自然科学版）》2004年第2期25-27,共3页Journal of Tianjin Normal University：Natural Science Edition

基　　金：湖北省科技厅科技攻关项目(2003AA101C21)

摘　　要：对测定核黄素常用的3种方法,即高效液相色谱法、荧光光度法和分光光度法进行比较.结果表明,对于标准溶液3种方法均具有良好的线性关系和重现性,但在酵母提取液中,高效液相色谱法和分光光度法的回收率较差,测定准确率低.荧光光度法测定准确性高,稳定性强,为测定酵母胞内核黄素的最佳方法.Intracellular riboflavin in both standard solution and yeast extract was determined by using high performance liquid chromatography(HPLC), fluorometry and spectrophotometry, respectively. The results showed that the three methods have good linearity and repetition for standard samples. But for years extract, the recovery, veracity and recurity of fluorometry were better than that of other two methods. Thus, the fluorometry was regarded as a suitable method for determining intracellular riboflavin of yeast.

关 键 词：酵母 胞内核黄素 测定方法 

分 类 号：Q503[生物学—生物化学] TQ924[轻工技术与工程—发酵工程]