ELISA法和HPLC法检测粮油食品中黄曲霉毒素B_1含量的比较研究  

Comparison of ELISA with HPLC in detection of aflatoxin B_1 in corn and vegetable oil foods

作　　者：蔡正森[1] 钟文辉[2] 张东升[1] 赵春城[1] 龚燕[1] 蔡建荣[1] 赵晓联[1] 

机构地区：[1]江苏省微生物研究所有限责任公司,无锡214063 [2]南京师范大学化学与环境科学学院,江苏210097

出　　处：《中国卫生检验杂志》2004年第3期302-304,共3页Chinese Journal of Health Laboratory Technology

基　　金：科技部十五科技攻关项目 (2 0 0 1BA80 4A1 4 )

摘　　要：〔目的〕研究和评价ELISA和HPLC两种方法检测粮油食品中黄曲霉毒素B1 之间的差异。〔方法〕用两种检测方法同时检测大量样品 ,并做加标回收试验。〔结果〕ELISA法和HPLC法相对误差平均值为 5 .2 5 %。在AFB1 浓度为0 .5～ 12 .5ppb时 ,ELISA法回收率为 3 5 .2 1%～ 15 5 .13 % ,HPLC回收率为 3 3 .74%～ 75 .70 %。〔结论〕ELISA法比HPLC法操作简便 ,快捷 。〔Objective〕 To compare ELISA with HPLC in detection of aflatoxin B 1 in corn and vegetable oil foods.〔Methods〕 Aflatoxin B1 was detected in various samples by ELISA and HPLC,respectively.Meanwhile,the spiked recovery test was performed.〔Results〕 The recovery rate was 35.21%～155.13% by ELISA but 33.74%～75.70% by HPLC.〔Conclusions〕 ELISA is more convenient and rapid than HPLC.

关 键 词：ELISA法 HPLC法 粮油食品 黄曲霉毒素B1 毒性 

分 类 号：O657.71[理学—分析化学] R446.61[理学—化学]