ELISA法检测红曲中的黄曲霉毒素B_1  

Determination of Aflatoxin B_1 in Red Leaven by ELISA

作　　者：龚燕[1] 蔡建荣[1] 赵晓联[1] 黄国水 孙开禧 

机构地区：[1]江苏省微生物研究所,江苏无锡214063 [2]福建省连江县卫生防疫站,福建连江350500

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第4期10-12,共3页Journal of Wuxi University of Light Industry

基　　金：科技部农业成果转化资金项目(02EFN213)资助课题.

摘　　要：探讨了红曲中黄曲霉毒素B1的提取方法,发现在传统提取方法甲醇/水溶液(体积比1∶1)直接提取的基础上加氯仿进行液液萃取后,进行ELISA法测定其含量,可以消除假阳性,同时提高了检测灵敏度.在加标质量分数分别为2.5,5,10μg/kg时,加标回收率达到101%～146.8%,方法的重复性较好,变异系数小于13%,样品最低检测限达2.5μg/kg.The extraction method of aflatoxin B_(1) in red leaven was studied in this paper.The results showed that 10 mL methanol and water solution of total 25 mL extract extracted by chloroform can eliminate negative error and improve the detection sensitivity. The recoveries of aflatoxin when B_(1 )spiked at 2.5～10 μg/kg could be at the range of 101%～146.8% and the relative deviation is less than 13%.The detection low limit was 2.5 μg/kg.

关 键 词：红曲 提取方法 ELISA 黄曲霉毒素B1 

分 类 号：TS201.6[轻工技术与工程—食品科学]