球孢白僵菌产几丁质酶液态发酵研究  

Studies on Liquid State Fermentation of Tricoderma Sp. T6

作　　者：张洁[1] 张敏[1] 吴茜茜[1] 刘斌[1] 

机构地区：[1]合肥学院生物科学与技术系,安徽合肥230022

出　　处：《合肥学院学报（自然科学版）》2004年第1期53-56,108,共5页Journal of Hefei University :Natural Sciences

基　　金：安徽省教育厅重点建设课程项目(No.200167)资助;安徽省优秀青年科技基金(No.04043051)

摘　　要：对球孢白僵菌Bb174产几丁质酶的液态发酵条件进行了试验研究。选择麸皮和蚕蛹粉作为培养基的基本成分。以不同比例的麸皮和蚕蛹粉进行试验,结果显示:麸皮和蚕蛹粉分别为5％,培养温度28℃,接种量10％,装液量10mL,起始pH6.0,添加0.3％NaNO_3对产儿丁质酶有利,最高酶活力为4664U/mL。Studies on liquid state fermentation of Tricoderma sp. T6 are made. Wheat bran and the powder of the silkworm pupae are selected as the basic media component. Both of the inverse proportion of wheat bran and the powder of the silkworm pupae fixed by experiments are 5%. The results shows that the optimal conditions are as follows: 28℃, inoculum size 10%, loading size 10ml, initial pH6.0. It is beneficial for producing chitinase by adding 0.3% NaNO_3. The maximum enzyme activity is 4664U/mL.

关 键 词：球孢白僵菌 几丁质酶 液态发酵 培养基 可溶性蛋白质 培养温度 

分 类 号：TQ925[轻工技术与工程—发酵工程]