Reversal of Multidrug Resistance by Neferine in Adriamycin Resistant Human Breast Cancer Cell Line MCF-7/ADM  

甲基莲心碱对耐阿霉素人乳腺癌细胞多药耐药性的逆转(英文)

作　　者：曹建国[1] 唐小卿[1] 周虹[1] 彭波[1] 

机构地区：[1]衡阳南华大学医学院肿瘤研究所,421001

出　　处：《The Chinese-German Journal of Clinical Oncology》2004年第2期93-96,125,126,共6页中德临床肿瘤学杂志（英文版）

摘　　要：Objective: To study the reversal effect of neferine on adriamycin (ADM) resistant human breast cancer cell line MCF-7/ADM. Methods: The cytotoxic effect of Nef or ADM was determined by 3-[4, 5-dimethylthiazol-2.-yl], 5-diphenyl tetraxolium bromid (MTT) assay. Apoptosis and the expression of P-glycoprotein (P-gp) were detected by flow cytometry (FCM). The intracellular ADM concentration was measured by HPLC. Results: Nef at 1, 5, 10 mol/L decreased the IC50 of ADM to MCF-7/ADM from 11.63 g/mL to 4.59, 2.44, 0.27 g/mL respectively. MCF-7/ADM could resist the apoptosis induced by ADM while Nef (1-10 mol/L) could augment ADR-mediated apoptosis. Nef (10 mol/L) increased the accumulation of ADM up to 2.88 fold in MCF-7/ADM but not in sensitive cells MCF-7/S and reduced the expression of P-gp in MCF-7/ADM cells. Conclusion: Nef can circumvent multidrug resistance (MDR) of MCF-7/ADM cells and the mechanism was associated with the increase of intracellular accumulation of ADM and the reduced expression of P-gp in MCF-7/ADM cells.

关 键 词：NEFERINE multidrug resistance ADRIAMYCIN MCF-7/ADM cells 

分 类 号：R737.9[医药卫生—肿瘤]