膀胱肿瘤6号染色体长臂的杂合性缺失研究  

Loss of heterozygosity of microsatellite DNA on 6q in bladder tumor

作　　者：成凡[1] 楚雍烈[1] 贺大林[2] 杨林[2] 陈萍[1] 杨娥[1] 刘湘[1] 

机构地区：[1]西安交通大学医学院微生物教研室 [2]西安交通大学第一附属医院泌尿外科,陕西西安市710061

出　　处：《中国康复理论与实践》2004年第5期279-280,共2页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探讨 6号染色体长臂的杂合性缺失 (LOH)与膀胱肿瘤的关系。方法应用 6q2 1区域附近D6S40 4、D6S43 4微卫星标志 ,以PCR SSLP 银染法对 3 1例膀胱肿瘤患者的肿瘤组织进行杂合性缺失的研究。结果膀胱肿瘤组织D6S40 4LOH发生率为 3 5 5 %,D6S43 4LOH发生率为 2 2 6%。结论 6q2 1区域附近可能存在与膀胱肿瘤相关的肿瘤抑制基因。ObjectiveTo investigate the loss of heterozygosity (LOH) on 6q in bladder tumor.MethodsD6S404 and D6434 microsatellite markers near 6q21 were tested by PCR-SSLP-stain method on tumor DNA from 31 cases of bladder tumor.ResultsAmong these 31 cases of bladder tumor,LOH was detected in tumor tissues on site for D6S404 (35.5%) and D6S434(22.6%).ConclusionOne or more tumor suppressor gene near 6q21 maybe relevant for the development of bladder tumor.

关 键 词：膀胱肿瘤 杂合性缺失 6号染色体长臂(6q) 

分 类 号：R737.14[医药卫生—肿瘤]