低盐压力下缢蛏血淋巴蛋白的比较蛋白质组学研究  

Comparative Proteomics of Proteins in Sinonovacula constricta Hemolymph under Low Salinity

作　　者：张鹭[1] 陈寅山[2] 彭宣宪[3] 

机构地区：[1]齐齐哈尔大学生命科学院,齐齐哈尔161005 [2]福建师范大学生物工程学院,福州350007 [3]厦门大学生命科学院,厦门361005

出　　处：《吉林农业大学学报》2004年第3期272-274,279,共4页Journal of Jilin Agricultural University

基　　金：福建省教育厅资助项目(A类)(JA02179)

摘　　要：应用双向聚丙烯酰胺凝胶电泳技术及图像分析技术寻找缢蛏在低盐环境压力下血淋巴内40kD以下小分子蛋白质组差异,制成肽质指纹图谱,MALDI-TOF-MS测序,通过数据库检索快速鉴定蛋白质或多肽。研究结果表明:低盐压力下,缢蛏血淋巴内有多种蛋白质发生变化,共鉴定出包括磷脂酶A2(Phospho lipaseA2)、锌指蛋白(ZincFingerProtein)等6种蛋白或多肽,其功能涉及营养、基因表达及蛋白合成调控等方面,说明其血淋巴在缢蛏的防御体系中具有关键性作用。The differences of the whole protein(under 40 kD) expression profiles in Sinonovacula constricta hemolymph after shot-term treatment of low salinity are detected using two-dimensional electrophoresis and image analysis software,The peptide mass map is obtained with MALDI-TOF-MS,A novel protein or polypeptide is identified by database retrieval.Several proteins or polypeptides in S. constricta hemolymph change under low salinity stress, including Phospholipase A2, Zinc finger protein and so on, which are of multiple functions such as nutrition, regulatory gene expression and protein synthesis, and so on. It′s obvious that hemolymph is critical in S. constricta defense mechanism.

关 键 词：缢蛏 血淋巴 双向电泳 质谱 低盐 蛋白质 

分 类 号：Q51[生物学—生物化学] S185.34[农业科学—农业基础科学]