金雀异黄素对乳腺癌细胞iNOS表达影响  

Effect of genistein on iNOS expression and anticancer in human breast carcinoma cells

作　　者：刘颖[1] 卢琳[1] 董继生[1] 迟晓星[1] 李百祥[2] 崔洪斌[2] 

机构地区：[1]哈尔滨商业大学生物工程系,哈尔滨150076 [2]哈尔滨医科大学公共卫生学院毒理学教研室

出　　处：《中国公共卫生》2004年第4期395-396,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金项目 (30 1 0 0 1 51 );黑龙江省自然科学基金项目 (D0 1 - 2 4 )

摘　　要：目的　研究金雀异黄素 (Genistein ,Gen)对人乳腺癌细胞MCF - 7增殖和诱导型一氧化氮合酶 (iNOS)基因表达的影响以及两者之间的关系。方法　采用MTT试验和3 H -TdR掺入法观察Gen抑制乳腺癌细胞的生长 ,免疫组化及RT -PCR方法检测iNOS的表达。结果　Gen显著抑制MCF - 7细胞的增殖 ,细胞上清液中NO水平有升高的趋势 ,并显著增加iNOS的蛋白表达 ,对iNOSmRNA的表达无显著影响。结论　Gen抑制乳腺癌细胞增殖的机制之一可能与其上调iNOS基因表达有关。Objective To study the genistein on the effect of proliferation and the iNOS expression of human breast carcinoma MCF-7 cells.Methods MTT was used to investigate the effects of genistein in breast cancer cell proliferation.Immunocytochemstitry and RT-PCR were used to observe the effects of genistein on iNOS gene expression.Results Genistein inhibited the proliferation of tumor cells significantly and increased iNOS protein expression.Conclusion Proliferatory inhibition of human breast carcinoma cells after being treated with genistein may be due to the up-regulation expression of iNOS.

关 键 词：金雀异黄素 乳腺癌细胞 诱导型一氧化氮合酶 一氧化氮 

分 类 号：R181.38[医药卫生—流行病学]