微生物发酵法生产谷胱甘肽  

Progress in Glutathione Production by Microbial Fermentation

作　　者：陈坚[1] 卫功元[1] 李寅[1] 堵国成[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《无锡轻工大学学报（食品与生物技术）》2004年第5期104-110,共7页Journal of Wuxi University of Light Industry

基　　金：江苏省高校高新技术产业发展项目(编号:JH02 101);高等学校优秀青年教师教学科研奖励计划资助课题.

摘　　要：谷胱甘肽是一种重要的生理活性肽类物质,对于维持生物体内合适的氧化还原环境起着关键作用,在临床医药、食品工业和体育运动领域具有广阔的应用前景.微生物发酵法是目前谷胱甘肽生产的主要方法.本文综述了直接发酵法生产谷胱甘肽中高产菌株选育、发酵过程优化与控制以及基因工程菌在谷胱甘肽生产中的应用潜力,最后对发酵法合成谷胱甘肽需要解决的问题进行了简单展望.Glutathione (γ-glutamylcysteinylglycine, GSH is the reduced form), the most ubiquitous low molecular weight non-protein thiol compound, is widely distributed in almost all the aerobic organisms. Being one of the important physiological active peptides, GSH plays many physiological functions in the cells, for instance, maintenance of normal redox potential in the cells, radical scavenger, detoxification of various cytotoxic compounds, antioxidant, and so on. As glutathione has wide application potential in medicine, food, athletic sports and so on, the commercial demand for GSH has been expanding. The main technique for GSH preparation at present is by biotechnological method, which comprises enzymatic transformation and microbial fermentation. In this article, GSH production by fermentative approach within the recent 30 years was reviewed. The screening of strains for high GSH production, optimization and control of the bioprocess, and the application potential of genetic bacterium for GSH overproduction by fermentation were described in detail. In the end, the problems to be solved and the foreground for biotechnological production of GSH were briefly prospected.

关 键 词：谷胱甘肽 发酵 生产 进展 

分 类 号：TQ929[轻工技术与工程—发酵工程]