利用马铃薯渣和黑淀粉发酵柠檬酸的菌种选育  

Breeding of the Citric Acid Producing Strains Utilizing Potato Residue and Black Potato Starch

作　　者：杨庆文[1] 孙文敬[1] 谢红[1] 赵峰梅[1] 郭金权[1] 王慕华[1] 

机构地区：[1]山西省生物研究所,山西太原030006

出　　处：《山西农业科学》2004年第4期57-59,共3页Journal of Shanxi Agricultural Sciences

摘　　要：从91株黑曲霉中筛选出菌株A-44,经Co60-γ射线诱变,获得了一株利用马铃薯精淀粉下脚料深层发酵柠檬酸的变异株γ-115。当发酵培养基中马铃薯渣13%、黑淀粉10%时,摇瓶产酸达132～149mg/ml,对淀粉的转化率达83.50%～94.30%;当发酵培养基中马铃薯渣10%、黑淀粉6%时,30L发酵罐产酸达72～84mg/ml,对淀粉的转化率达81.40%～94.32%。The citric acid producing mutant γ-115 which could utilize potato residue and black potato starch was obtained, after treating the strain A-44 with Co~60 radiation which was selected from 91 strains of Aspergillus niger. With the fermentation medium containing 13% of potato residue and 10% of black potato starch, the citric acid yield in the shaking flask was 132～149mg/ml and the conversion rate was 83.50%～94.30%. With the fermentation medium containing 10% of potato residue and 6% of black potato starch, the citric acid yield and the conversion rate in a 30L fermentor was 72～84mg/ml and 81.40%～94.32% respectively.

关 键 词：马铃薯渣 黑淀粉 发酵 柠檬酸 菌种选育 原料 

分 类 号：TQ921.1[轻工技术与工程—发酵工程]