人类植入前胚胎形态与染色体异常关系初探  

Preliminary Analysis of Relationship Between Morphology and Chromosome Abnormality in Human Preimplantation Embryos

作　　者：方丛[1] 庄广伦[1] 周灿权[1] 徐艳文[1] 钟依平[1] 彭文林[1] 詹前胜[1] 

机构地区：[1]中山大学附属第一医院生殖医学中心,广东广州510080

出　　处：《中山大学学报（医学科学版）》2004年第5期458-461,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30200301);广东省卫生厅基金资助项目(A2001161)

摘　　要：【目的】探讨人类植入前胚胎发育及形态与染色体异常之间的关系。【方法】对6例染色体异常患者的67个胚胎进行植入前遗传学诊断,对其进行形态评定,并用荧光原位杂交方法对胚胎进行染色体分析,分为染色体正常胚胎组及异常组。【结果】分析的67个胚胎中,染色体正常16个,异常51个。染色体正常及异常的胚胎在取卵后72h发育至6细胞以上的比率分别为68.75%,62.75%;正常与异常的胚胎中碎片均<20%的胚胎分别占100%、94.12%;胚胎活检后继续发育率分别为73.33%,79.54%;差异均不具显著性。【结论】用荧光原位杂交方法可对植入前胚胎进行染色体分析并研究早期胚胎形态与染色体异常之间的关系。To analyze the relationship between morphology and chromosome abnormality in the human preimplant ation embryos.Preimplantation genetic diagnosis were performed in 67embryos from 6patients with chromosome abnormality.Embryos morphology were assessed and fluorescence in situ hybridization was used to analyze the chromosome in human preimplantation embryos.Emb ryos were divided into normal and abn ormal chromosome group.Among 67embryos,16were normal and 51were abnormal.The percentages of t he≥6cells embryos in the normal and abnormal were 68.75%and 62.75%,respectively.The percentages of fragment <20%embryos were 100%an d 94.12%,respectively.The percent ages of further cleavage were 73.33%and 79.54%,respectively.The differences were not significant between the two groups.[Conclusion]Fluorescence in situ hybridization can be used to analyze the chromosome and the relationship between morphology and chromosome abnormality in t he human preimplantation embryos.

关 键 词：染色体异常 正常 胚胎活检 染色体分析 荧光原位杂交 发育 植入前遗传学诊断 植入前胚胎 形态 人类 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R714[医药卫生—基础医学]