多肽的电泳分离  

The Electrophoretic Separation of Low Molecular Weight Polypeptide

作　　者：左爱军[1] 梁东春[1] 郭刚[1] 王宝利[1] 张镜宇[1] 

机构地区：[1]天津医科大学内分泌研究所,300070

出　　处：《天津医药》2004年第10期604-606,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金重点项目 (项目编号 :033801611)

摘　　要：目的 :对4种PAGE方法进行比较 ,选择出适用于分离小分子质量多肽的电泳方法。方法 :用常规SDS_PAGE、低分子质量Tricine_SDS_PAGE、多肽SDS_PAGE以及含尿素的多肽SDS -PAGE4种方法对相同的样品进行电泳分离 ,并对分离效果进行比较。结果 :常规SDS_PAGE不适合分离小分子质量多肽 ,Tricine_SDS_PAGE对分子质量小于10ku的样品效果不佳。多肽SDS_PAGE能分离分子质量在5～20ku的多肽 ,含尿素的多肽SDS_PAGE对分子质量小于5.0ku的多肽也能很好的分离。结论 :含尿素的多肽SDS_PAGE对小分子质量多肽的分离效果最好 ,可根据实验的需要选择适合的电泳方式。Objective:To select an ideal method for the separation of polypeptide,and compare the four SDS_PAGE methods in separating low molecular weight polypeptide.Methods:Identical samples of low molec-ular protein marker and polypeptide molecular marker were electrophoresised by conventional SDS_PAGE,Tricine_SDS_PAGE,SDS_PAGE for polypeptide and the SDS_PAGE containing urea.The separating effects of these four methods were compared.Results:Routine SDS_PAGE was not adaptable for separating low molecular weight peptide.Low molecular weight peptide(<10ku)was not separated by Tricine_SDS_PAGE well.Low molecular weight polypeptide(5～10ku)could be separated by polypeptide SDS_PAGE.If urea were applied,polypeptide SDS-PAGE could separate the peptide lower than5ku.Conclusion:Containing urea polypeptide SDS-PAGE had the highest ability of separating low molecular weight peptide.The suitable method should be applied depends on the needs of the experiment.

关 键 词：多肽 SDS-PAGE 电泳分离 效果不佳 小分子 低分子 常规 分子质量 

分 类 号：R446[医药卫生—诊断学] R284[医药卫生—临床医学]