常见食用菌中转基因成分定性PCR检测方法的建立  

Development of PCR Detection Method for Geneticcally Modified Component in Familiar Edulis Fungi

作　　者：陈文炳[1] 江树勋[1] 邵碧英[1] 李寿崧[1] 朱晓南[1] 王泽生[2] 廖建华[2] 

机构地区：[1]福建出入境检验检疫局,福建福州350003 [2]福建省轻工业研究所双孢蘑菇菌种研究推广站,福建福州350005

出　　处：《食品科学》2004年第10期206-210,共5页Food Science

基　　金：国家质量监督检验检疫总局科研项目(2002IK084;2003IK046);福建省科技重大项目(2001H011);福建省青年科技人才创新项目(2001J040)

摘　　要：将食用菌转基因研究用的质粒载体(p301-bG1)DNA,添加到19种常见食用菌样品中,作为模拟阳性样品,从中提取出DNA用于PCR分析,建立了常见食用菌中3个大小分别为165、398、599bp的外源基因(NOS、BAR、GUS)的特异性DNA片段的定性PCR检测方法,还进行了二重与三重PCR分析,并通过不同的模板DNA浓度对PCR扩增结果的影响,分析了PCR检测的灵敏度。DNA extracted from plasmid vector (p301-bG1) used in transgenic research of familiar edulis fungi was added into19 familiar edulis fungy samples as simulated positive samples. The qualitification PCR was used to detect genetically modifiedcomponent in edulis fungi in present study. DNA extracted from samples by CTAB method was amplified by single PCR andmultiplex PCR. We developed the PCR detection method for the target DNA fragments with the size of 165bp, 398bp and599bp of three transgenes, i.e. NOS, BAR and GUS, respectively. Effect of different DNA concentration of positive sample (p301-bG1 plasmid) to PCR analysis was also analyzed.

关 键 词：食用菌 转基因成分 PCR检测 

分 类 号：TS207[轻工技术与工程—食品科学]