离子交换法从酶解液中提取阿魏酸  

Extraction of Ferulic Acid from Enzymatic Hydrolysate by Ion-exchange Adsorption

作　　者：刘子立[1] 欧仕益[1] 黄雪松[1] 罗艳玲[1] 谢静[1] 徐海英[2] 蒋笃孝[1] 

机构地区：[1]暨南大学食品科学与工程系,广州510632 [2]湛江海洋大学,广东湛江524025

出　　处：《中药材》2004年第12期910-913,共4页Journal of Chinese Medicinal Materials

基　　金：广州市科技攻关项目编号 :2 0 0 3Z3 10 0 61

摘　　要：以麦麸酶法降解液为原料 ,采用D2 0 1大孔树脂对阿魏酸进行分离提纯 ,探讨了上柱工艺条件和洗脱条件 ,确定最佳离子交换操作工艺 :室温下 ,料液阿魏酸浓度在 2 0 0 0mg/L～ 30 0 0mg/L之间 ,pH值 9 0 ,上柱流速 1ml/min ,洗脱剂配比 (V/V)为无水乙醇∶水∶盐酸 =6 0∶36∶4 ,洗脱流速为 1ml/min。在该操作工艺条件下 ,阿魏酸收率达 97%以上 ,且纯度大大提高。Anion-exchange resin,D201 was used to prepare ferulic acid from the enzymatic hydrolysate of wheat-bran and the influence of adsorption and desorption operation was researched The optimal technology is as follows:under the room-temperature,the concentration of ferulic acid in the extraction solution is 2000 mg/L～3000mg/L,pH and flow velocity were kept around 9 0 and 1 ml/min respectively;elution solution is composed of ethanol:water:HCl in ratio 60∶36∶4(v/v),and the velocity of elution is 1ml/min Under optimal conditions,the recovery percent of ferulic acid from enzymatic hydrolysate exceed 97% and the purification production is in evidence

关 键 词：阿魏酸 无水乙醇 大孔树脂 室温 洗脱剂 分离提纯 麦麸 离子交换法 收率 工艺条件 

分 类 号：R284[医药卫生—中药学] R286.0[医药卫生—中医学]