香菇单核原生质体制备和再生菌丝交配型测定  

PREPARATION AND MATING TYPE ANALYSIS OF PROTOPLASTED MONOKARYON IN Lentinus edodes

作　　者：潘迎捷[1] 伯海英[2] 沈宗英 

机构地区：[1]上海市农业科学院食用菌研究所,上海201106 [2]华东师范大学生物系,上海200062

出　　处：《上海农业学报》1993年第3期11-14,共4页Acta Agriculturae Shanghai

摘　　要：从9个香菇野生种和栽培种中制备出不同比例的单核原生质体再生菌丝(37％～85％)。溶壁酶浓度、酶解时间和菌丝生理状况对单核原生质体形成无明显影响。在9个单核原生质体再生菌丝中,有5个测出两种交配型(AxBx,AyBy),比例从2:1～10:1不等;有4个只测出一种交配型(AxBx)。产生这一现象的原因与不同交配型的单核原生质体的再生率和菌丝生长速度有关。在交配型等位基因的测定中,9个菌种测出12个A因子和12个B因子,交配型等位基因的重复频率为14.1％。High proportion (37％～85％) of protoplasted monokaryons(PM) were obtained in all the 9 test strains of L. edodes. The concentration of lytic enzyme, lysing time and the age of mycelium hadn't obvious effect on the preparation of PM. Two kinds of mating types(AxBx,AyBy) in each of 5 strains were obtained. The ratio of AxBx: AyBy was from 2:1,to 10:1. However, only one kind of mating type in each of the other 4 strains was obtained. It possibly resulted from the different regeneration rates and growth rates in different mating types of PM. 12 factors A and 12 factors B were determined during the mating of 14 model mating types of PM. from 9 strains. The repetition frequency of alleles in mating types of these strains was about 14.1％.

关 键 词：香菇 单核原生质体 交配型 测定 

分 类 号：S646.120.3[农业科学—蔬菜学]