肝癌高发区广西隆安县乙型肝炎病毒株全基因序列分析  

Full-length sequence of hepatitis B virus isolated from high incidence hepatocellular carcinoma area-Longan county

作　　者：葛宪民[1] 方钟燎[2] 李丹亚[3] 赵连三[4] 

机构地区：[1]广西壮族自治区职业病防治研究所,南宁530021 [2]广西壮族自治区疾病预防控制中心 [3]广西医科大学第一附属医院 [4]四川大学华西医学中心第一附属医院

出　　处：《中华实验和临床病毒学杂志》2004年第3期272-276,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　探讨肝癌高发区广西隆安县乙型肝炎病毒 (HBV)株的全基因序列是否存在地域特殊性。方法　用套式PCR(nPCR)扩增该县HBV无症状携带者血清HBV全基因 ,用克隆测序法测序并做同源性对比。结果　本病毒株共 32 15个碱基 ,基因型为C ,血清型为adw。其P基因区有 4 0处发生碱基点突变 ,导致 11个氨基酸改变 ;S基因区的前S1、前S2和S基因分别有 11、2和 3个碱基点突变 ,分别导致 3、1、1氨基酸改变 ,未发现“a”区突变 ;X基因区共有 6个碱基点突变 ,导致 4个氨基酸改变 ,其中出现能影响e抗原表达的双突变 (nt 176 2A→T、176 4G→A) ;未发现前C区基因突变 ,C基因有 13个碱基发生突变 ,导致 2个氨基酸改变。本病毒株与越南北部病毒株高于同为C基因型的上海株、北京株、西藏株。结论　未发现肝癌高发区的本例HBV基因C型的隆安株 ,基因虽有肝癌高发性 ,但无地域特殊性。Objective To understand full-length sequence of HBV isolated from high incidence hepatocellular carcinoma area-Longan county, Guangxi Methods The nested polymerase chain reaction (nPCR) was used for amplifying the whole HBV DNA in sera of asymptomatic carriers The products were sequenced by clone sequencing and homological analysis Results This isolate contained 3 215 bases The genotype was C and the serotype was adw There were 40 point mutations in polymerase gene which made 11 amino acids change There were 11,2 and 3 point mutations in PreS1,PreS2 and S gene respectively which made 3,1,1,amino acids change Six point mutations including the double mutations(nt 1762 A→T、1764G→A)were found in X gene leading to 4 amino acids change There were 13 point mutations in C gene which made 2 amino acids change No mutation was found in a determinant and Pre C The isolate was quite close to the isolate from Vietnamese in evolution while far from the genotype C isolates from Shanghai, Beijing and Tibet Conclusion No special sequence was found in the isolate from high incidence hepatocellular carcinoma area-Longan county, Guangxi

关 键 词：肝癌 高发区 改变 乙型肝炎病毒 点突变 HBV C基因 病毒株 氨基酸 上海株 

分 类 号：R735.7[医药卫生—肿瘤]