微生物发酵生产十一烷1,11二羧酸的研究  

STUDIES ON MICROBIAL PRODUCTION OF UNDECANE 1,11-DICARBOXYLIC ACID FROM N-TRIDECANE

作　　者：陈远童[1] 郝秀珍[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》1989年第3期241-245,共5页Chinese Journal of Biotechnology

摘　　要：从热带假丝酵母(Candida tropicalis)T25-14经过紫外线和亚硝酸的多次诱变,获得4株产十一烷1,11二羧酸(DC_(13))较多的突变株,其中最优的NP-159株以20%(V/V)正十三烷(nC_(13))为碳源摇瓶发酵4天,DC_(13)达80g/L左右。在16L罐上,以30%(V/V)nC_(13)发酵6天,DC_(13)高达139g/L,回收残烃后,对nC_(13)的转化率为80%以上。后处理收率为78.9%,DC_(13)的纯度为95.3%。Treatment of mutant, Candida tropicalis T_(25-14), with ultraviolet radiation and selection by indication medium containing alkane obtained a mutant UP-3-24 which yield of dioie acid was 20% higher than parent strain. Further treatment of mutant UP-3-24 with sodium nitrite and selection obtained a better mutant NP-159 which yield of dioic acid was 30% higher than parent strain UP-3-24. The better condition of cultivation for undecane 1,11-dicarboxylic acid (DC_(13)) production from n-tridceane (nC_(13)) by mutant NP-159 was investigated by using sucrose as carbon source for the growth. On shaking flask, under optimum condition where the culture medium contained 20%(V/V) nC_(13), 2%(W/V) sucrose, inorganic salts and growth factors and pH was adjusted to 7.5 with 6mol/L NaOH at 24 h intervals, the amount of the product accumulated was more than 80g per liter of the medium for 4 days. On 16 liter fermenter testing, under the optimum condition where the culture medium contained total 30%(V/V)nC_(13), pH of the course of fermentation was maintained range 7.3—7.5, at 28—30℃, the highest level of DC_(13) production was obtained after 6 days and the amount of DC_(13) accumulated was 139g per liter of the mudium. After received residual nC_(13), the consumption rate of nC_(13) was about 80%. The product from nC_(13) was analysed by gas-chromatograph the purity was 95.3%. The mutant strain NP-159 was still strong and healthy without recovery after subculture for years on malt extract-agar slant, the production of DC_(13) was also as high as usual. Therefore, mutant strain NP-159 could be comfirmed as stable and high producer for the production of DC_(13) in industry.

关 键 词：DC13 热带假丝酵母 发酵生产 

分 类 号：TQ921[轻工技术与工程—发酵工程]