鹿肉酶水解液中游离氨基酸的高效液相色谱分析  

High performance liquid chromatography of free amino acid in the deer enzymatic hydrolysate

作　　者：高贵[1] 张作明[1] 韩四平[1] 翁梁[1] 李媛媛[1] 马吉胜[1] 冯雁[1] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,吉林长春130023

出　　处：《中国生化药物杂志》2005年第1期32-33,共2页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的应用高效液相色谱法对鹿肉酶水解液中游离氨基酸进行检测。方法以AQC为衍生化试剂,采用柱前衍生化技术、紫外检测考察了氨基酸衍生和液相分离过程中的各种影响因素,检测鹿肉酶水解液中各种游离氨基酸含量。结果鹿肉酶水解液中蛋白质含量为83.2 8% ,总游离氨基酸含量为4 8.79mg/g。结论柱前衍生高效液相色谱法可用于检测鹿肉酶水解液中游离氨基酸含量。PurposeThe free amino acid in deer, which is hydrolysed by proteinase, is determined by high performance liquid chromatography (HPLC).Methods6-amino quinoly-N-hydroxy succinimidyl carbamate (AQC) is used as a derivation reagent. The factors that influence amino acid derivation and separation are examined through the pre-column derivation technique and UV detector.ResultsThe concentration of protein and total free amino acid is 83.28% and 48.79 mg/g, respectively.ConclusionPre-column derivation technique provides a simple and feasible way in detecting the free amino acid in the deer hydrolysate and offers some basic information on the deer hydrolysate application.

关 键 词：鹿肉 酶水解 柱前衍生 氨基酸 

分 类 号：Q556.03[生物学—生物化学]