沪酿3.042米曲霉紫外诱变及高活力蛋白酶菌株选育  

UV mutagenesis of aspergillus oryzae Shanghai brewing 3.042 and the screening of a highproteinase-producing strains

作　　者：陈亚光[1] 高朝辉[1] 陈勇[1] 王璐[1] 王艳珍[1] 逯家辉[1] 腾利荣[1] 孟庆繁[1] 

机构地区：[1]吉林大学生命科学学院,吉林长春130012

出　　处：《中国调味品》2005年第3期15-18,共4页China Condiment

基　　金：全国教育科学"十五"规划课题(DIA030165);吉林省教育科学"十五"规划课题(B315041)。

摘　　要：采用沪酿3.042米曲霉的孢子为对象,对其孢子进行紫外诱变,利用酪蛋白平板对2261株再生株进行初筛,得到34株蛋白酶活力提高的菌株,复筛得到一株蛋白酶活力高且遗传稳定的突变株LY06,37℃培养44h产酶活力由 313U/g曲料高到 556U/g曲料,为原菌株的 1 78 倍,传代培养15次,蛋白酶活力基本保持稳定,没有明显的下降。Adopted the spore of Aspergillus oryzae as experiment target,a UV induced mutagenesis was carried out,34 mutants producing high-output protease were selection from 2261 mutants by casein-degradating plate,calculating the activity of proteinase,a mutant LY06 producing high-output,stable protease was obtained.After growth at 37℃ for 44h, the activity of proteinase was increased from 313 U/g to 556U/g which is 1.78 times as high as original strain.the activity of proteinase were genetic stable after growing up to 15 generarions.

关 键 词：蛋白酶 沪酿3.042米曲霉 紫外诱变 

分 类 号：TS201.25[轻工技术与工程—食品科学]