pUC-T载体的构建  

Construction of pUC-T Vector

作　　者：吴亚锋[1] 梁东春[1] 郭刚[1] 张镜宇[1] 

机构地区：[1]天津医科大学内分泌研究所,300700

出　　处：《天津医药》2005年第3期159-160,共2页Tianjin Medical Journal

摘　　要：目的:构建具有较高连接效率和检出效率的克隆载体。方法:在质粒pUC18的多克隆位点内引入特异的DNA片段,酶切后得到3'末端具有突出T碱基的线性化pUC-T载体。结果:经连接效率实验,此载体具有很高的重组率。结论:成功获得了pUC-T载体;利用了质粒pUC18的多克隆位点,为亚克隆提供了丰富的酶切位点;具有蓝白斑筛选标记,提高了重组子的检出效率。Objective To get the cloning vector with high efficiency.Methods Insert a special DNA sequence into the multiple cloning site of pUC 18 and cut the product with restriction enzyme EclHK1 to get pUC-T vector. Results Experiment of Ligation proved the high recombinational efficiency of pUC-T vector. Conclusion Get the pUC-T vector and every product of enzyme cutting has the 3′-T protruding terminus with the multiple cloning site pUC-T has lots of choices in the subcloning strategy blue/white selection enhance the efficiency of the recombinant scanning.

关 键 词：UC 检出 T载体 质粒 克隆载体 白斑 筛选标记 酶切位点 重组率 重组子 

分 类 号：R574[医药卫生—消化系统] Q785[医药卫生—内科学]