紫外诱变原生质体选育D-核糖生产菌株  

Breeding of D-ribose producing mutants by protoplast mutagenesis of ultraviolet irradiation

作　　者：王慕华[1] 孙文敬[1] 郭金权[1] 赵峰梅[1] 秦丽 杨庆文[1] 

机构地区：[1]山西省生物研究所,太原030006 [2]郑州市生物化工厂,郑州450001

出　　处：《工业微生物》2005年第1期24-27,共4页Industrial Microbiology

基　　金：山西省科技攻关项目(NO.012055)

摘　　要：以D核糖产生菌枯草芽孢杆菌(Bacillussubtilis)B941为出发菌株,采用紫外诱变原生质体的方法,获得了4株可以在含有6.0%D核糖的培养基上生长的D核糖高产菌株,其摇瓶发酵产糖达55.0g/L左右。通过摇瓶发酵试验,研究了D核糖高产菌株Buvp24的遗传稳定性。研究结果表明,经多次传代,菌株Buvp24的发酵产糖能力及对发酵产物D核糖的耐受性没有改变,有望应用于工业生产中。The D-ribose producing strain Bacillus subtilis B941 was selected as the parent strain. The protoplast of B. subtilis was mutagenized by ultraviolet irradiation, and four mutants with high D-ribose producing ability were obtained. The mutants could grow on the culture medium with 6.0% D-ribose, and produced about 55.0g/L D-ribose in the shaking flask. The hereditary stability of the mutant Buvp-24 was investigated in the shaking flask, and the results showed that the mutant D-ribose producing ability and D-ribose endurance had no change over 9 generations. The mutant may be applied in the industrial fermentation process.

关 键 词：D-核糖 生产菌株 摇瓶发酵 高产菌株 紫外诱变 产生菌 原生质体 选育 遗传稳定性 发菌 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]