光学纯度L(+)-乳酸高产菌株的选育  

Screening of a optical purity L(+)-lactic acid high-production mutant strain

作　　者：吴石金[1] 周晓云[1] 郑怡[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2005年第2期119-123,共5页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(3940060)

摘　　要：从60份不同来源的土壤样品中筛选得到米根霉(Rhizopusoryzae)为出发菌株,通过紫外线和60Coγ射线诱变处理,运用推理育种技术,选育到一株有较高的乳酸脱氢酶活性和耐高渗的L(+)-乳酸高产突变株Rhi3-2.该菌株产L(+)-乳酸光学纯度高,最高产量为88.45g/L,较出发菌株提高91.24%,糖利用率提高17.87%,耗糖酸产率提高35.15%.菌株经8次传代培养,L(+)-乳酸产量下降10.7%,是一株性状较稳定可深入研究开发的优良菌株.Parent strain Rhizopus oryzae producing L(+)-lactic acid was isolated from 60 soil samples. A high-production mutant Rh3-2 was obtained from its parent strain Rhizopus oryzae by UV and ()(60)Coγ-ray treatment and rational screening. Mutant Rh3-2 is resistant to catabolic repression by high-concentration glucose and it produced higher optical purity L(+)-lactic acid. The L(+)-lactic acid prodution reached 88.45 g/L by flask culture, which was 91.24% higher than the parent strain. A desecend of only 10.7% in the L(+)-lactic acid yield of the mutant was observed after eight times of subculture. Therefore, the obtained mutant is a stable strain that is worthwhile to be studied further.

关 键 词：L(+)-乳酸 光学纯度 高产菌株 选育 ^60Coγ射线 乳酸脱氢酶活性 高产突变株 土壤样品 诱变处理 育种技术 传代培养 产量下降 优良菌株 研究开发 米根霉 紫外线 耐高渗 高产量 利用率 

分 类 号：TQ921.3[轻工技术与工程—发酵工程] TQ453.292