克劳氏芽孢杆菌S-4菌株固态发酵产碱性果胶酶  

Alkaline Pectinase Production by Bacillus clausii S-4 with Solid State Fermentaion

作　　者：张保国[1] 白志辉[2] 李祖明[3] 张国政[1] 张洪勋[2] 

机构地区：[1]天津科技大学食品科学与生物工程学院 [2]中国科学院生态环境研究中心,北京100085 [3]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《食品与发酵工业》2005年第3期8-11,共4页Food and Fermentation Industries

基　　金：国家 8 63计划资助项目 (No .2 0 0 1AA2 4 60 74)

摘　　要：对克劳氏芽孢杆菌(Bacillusclausii)S 4菌株产碱性果胶酶的固态发酵条件进行了优化,并对酶的部分性质进行了分析,结果表明,以甜菜粕为碳源和酶的诱导物,酵母膏和麸皮作氮源较适宜。固体培养基的组成:甜菜粕5 0g ,麸皮2 0 g ,KH2 PO40 0 15g ,MgSO4·7H2 O 0 2 5g ,Na2 CO3 0 12g ,水2 0mL ;培养温度4 0℃;发酵时间72h ;产酶率可达2 30 0u/g(甜菜粕)。该酶具有果胶水解酶和果胶裂解酶的活性,在pH 10 5 ,反应温度6 0℃时酶活力最高,在pH9 5～11 0范围内,温度4 0℃以下较稳定。1 0mmol/L的Ca2 + 、Tween 80和SDS对该酶有明显的激活作用,Mn2 + 、Cu2 + 、Zn2 + 对其有强烈的抑制作用。Fermentation conditions of the Bacillus clausii S-4 for production of alkaline pectinase and the properties of the enzyme were investigated. Sugar beet pulp was used as inducer and carbon source, and yeast extract and wheat bran were used as nitrogen sources. The optimal fermentation medium contained: sugar beet pulp 5.0 g, wheat bran 2.0 g, KH 2PO 4 0.015 g, MgSO 4·7H 2O 0.25 g, Na 2CO 3 0.12 g, water 20?mL. After inoculation, the medium were cultivated at 40℃ for 72 h, and the yield of alkaline pectinase was 2 300 u/g sugar beet pulp. The enzyme extract from the cultivated medium has pectin hydrolase and lyase activity. The highest activity of the enzyme is at pH 10.5 and 60℃. It is seen that Ca 2+ , Tween 80 and SDS enhance the activity of the enzyme, and Mn 2+ , Cu 2+ , Zn 2+ inhibit its activity.

关 键 词：碱性果胶酶 芽孢杆菌 Tween80 菌株 固态发酵条件 固体培养基 MgSO4 果胶裂解酶 Ca^2+ MN^2+ Cu^2+ ZN^2+ 甜菜粕 培养温度 发酵时间 反应温度 激活作用 抑制作用 诱导物 酵母膏 水解酶 酶活力 SDS 麸皮 碳源 产酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]