不同的碳源、氮源及碳氮比对微生物溶磷的影响  

Effect of C, N sources and m_C/m_N ratio on the phosphate solubilization of Aspergillus niger ML2 and ML4

作　　者：刘晓芳[1] 黄晓东[2] 孔健[3] 

机构地区：[1]山东商业职业技术学院,山东济南250103 [2]山东省生物药物研究院,山东济南250014 [3]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《山东大学学报（理学版）》2005年第2期121-124,共4页Journal of Shandong University(Natural Science)

摘　　要：利用液体发酵实验测定不同的碳源、氮源及碳氮比对两株黑曲霉ML2、ML4溶磷的影响.结果发现:当以NH+4为惟一氮源时,两株曲霉均表现出较高的溶磷活性,但当NH+4和NO3- 同时存在时,溶磷能力却大幅度下降.ML2、ML4在以葡萄糖为碳源时溶磷活性最高,溶磷量分别达到15 4 1.1mg L和16 6 8.2mg L .而以糖蜜为惟一碳源时,ML4的溶磷量只有4 1.2mg L .碳氮比对ML2、ML4的溶磷能力影响很大,随着碳氮比的增大,溶磷量随之升高,在mC∶mN =35∶1时达到最高,而后降低.在实验中还发现:菌株在溶磷过程中伴随着pH值的下降,但下降的程度与溶磷大小相关性较差.对其溶磷机制初步研究发现,溶磷菌株的溶磷效果主要依靠发酵液中的草酸和α酮戊二酸等有机酸的作用.The requirements of different carbon and nitrogen sources of Aspergillus niger ML2 and ML4 were studied for phosphate solubilization in this work. The result shows that when NH+_4 works as the sole nitrogen source, the ability of dissolving phosphate of both strains of Aspergillus niger is as well as each other. While NH+_4 and NO-_3 are used together, the ability goes down instead. Glucose was invested as the best carbon source, with which the efficiency of dissolving phosphate was increased distinctly to 1?541.1?mg/L and 1?668.2?mg/L respectively. However, the ability of ML4 to dissolve phosphate went down to 41.2?mg/L, when take gooey as the only carbon source. It shows that m_C/m_N ratio has a significant effect on the ability of dissolving phosphate, which goes up until the ratio got 35∶1. It is found that the pH value of the liquid fermentation has droped, but it has little relationship with the ability of dissolving phosphate.It is found that the mechanism of phosphate solubilization of the strains depend on oxalic acid and α-ketoglutaric acid produced in the liquid fermentation.

关 键 词：碳源 氮源 碳氮比 有机酸 

分 类 号：Q935[生物学—微生物学]