沙棘果原花青素的分离纯化研究  

Study on Isolation and Purification of Procyanidins from Seabuckthorn Fruit

作　　者：张驰[1] 徐晓云[1] 潘思轶[1] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2005年第5期183-185,共3页Food Science

基　　金：加拿大MRAC(MedicalResearchAdvisoryCommittee)项目(2002MRAC109)

摘　　要：用大孔树脂、SephadexLH-20凝胶色谱的方法和反相高效液相色谱分离纯化沙棘果中的原花青素。结果表明:大孔树脂层析法分离纯化沙棘果中的原花青素时使用50%的乙醇水溶液洗脱得到的洗脱液中原花青素的含量较高,达71.9%;将经过大孔树脂层析分离纯化的原花青素粗品经SephadexLH-20柱分离,50%乙醇作为流动相,原花青素粗品可纯化至95%以上。反相高效液相色谱分析发现,从SephadexLH-20洗脱下来的组分中含有原花青素的二聚体。The procyanidins from seabuckthorn fruit was isolated and purified with macroporous resin, Sephadex LH-20 and RP-HPLC. The result showed that procyanidins yield in elution aqueous was 71.9% while macroporous resin was eluted with 50% ethanol aqueous. The elution aqueous was further purified by Sephadex LH-20 with 50% ethanol/water(V/V) as mobile phase, procyanidins yield in elution aqueous was above 95%. The purified product was further analysed by RP-HPLC. It indicated that dimeric procyanidins were existed.

关 键 词：沙棘果 原花青素 分离纯化 大孔树脂 SEPHADEX LH-20 

分 类 号：TS201.2[轻工技术与工程—食品科学]