不同来源木瓜蛋白酶合成Aspartame前体能力的研究  

Ability of Pre-Aspartame Synthesis in Water-Ethyl Acetate Catalysed by Various Papain

作　　者：姚文兵[1] 吴梧桐[1] 范光斌[1] 黄岩山 许激扬[1] 金生浩[1] 严悦日[1] 

机构地区：[1]中国药科大学生物化学教研室

出　　处：《中国药科大学学报》1994年第2期113-115,共3页Journal of China Pharmaceutical University

基　　金：国家科学技术委员会资助

摘　　要：研究了Ｓｉｇｍａ公司、Ｍｅｒｃｋ公司和广州远天酶制剂厂生产的木瓜蛋白酶和从木瓜青果中提取的木瓜蛋白酶在水－乙酸乙酯两相反应系统中合成二肽甜味剂Ａｓｐｅｒｔａｍｅ前体（Ｐ－ＡＰＭ）的能力，并以ＨＰＬＣ（流动相为乙腈－水＝４５：５５，检测波长２６０ｎｍ，流速１ｍｌ／ｍｉｎ）和ＴＬＣ（硅胶ＧＦ＿（２５４）板，展开剂为正丁醇－醋酸－水＝３：１：１，显色剂为１％茚三酮丙酮液）对Ｐ－ＡＰＭ进行检测，实验结果表明，Ｐ－ＡＰＭ与以嗜热蛋白酶催化合成的Ａｓｐａｒｔａｍｅ中间产物极为相似。不同来源的木瓜蛋白酶由于其酶活力不同，对Ｐ－ＡＰＭ的合成和水解能力也有差异，选择国产的木瓜蛋白酶合成Ａｓｐａｒｔａｍｅ，为酶法合成二肽甜味剂提供了可探讨的途径。The ability of pre-aspartame synthesis in water-ethyl acetate two-phase system catalysed by different kinds of papains is studied. These papains are obtained from Sigma Chemical Co,E. Merck Darmstadt,GuangZhou Yuan Tian Enzyme Factory and isolated from natural sources [C. speciosa (Sweet) Nakai]by our laboratory. Pre-aspartames are analyzed directly by thin layer chromatography (silican gelGF254 in n-BuOH : AcOH :2O=3:1:1) and high pressure liquid chromatography (support :Shim-pack CLC ODS;gradient :acetonitrile :water=45:55; flow rate :1ml/min;detection :UV 260 nm). The results show that the pre-aspartame catalysed by papain is very similar to that which is catalysed by thermolysin and the papain which is obtained from Guangzhou Yuan Tian Enzyme Factory is proper for catalysing the. synthesis of pre-aspartame.

关 键 词：二肽甜味剂 木瓜 蛋白酶 酶法合成 

分 类 号：R914[医药卫生—药物化学]