ATM基因在髓性白血病原代细胞中的表达及其对基因治疗的意义  

Expression of ATM Gene in Myeloid Leukemia Primary Cells and Its Significance for Gene Therapy

作　　者：汤屹[1] 刘文励[1] 周剑锋[1] 徐慧珍[1] 路武[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《华中科技大学学报（医学版）》2005年第2期179-181,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No 39800149)

摘　　要：目的　研究在髓性白血病原代细胞和肿瘤细胞株中共济失调毛细血管扩张症突变基因(ATM) 的转录水平, 以确定反义封闭ATM基因治疗在白血病中应用的可行性。方法　利用半定量RT -PCR技术, 检测了正常人及31例髓性白血病患者和9种肿瘤细胞株的ATM mRNA表达量。结果　31例髓性白血病患者及9种肿瘤细胞株中ATMmRNA表达量普遍降低。结论　由于白血病患者中ATM mRNA表达量普遍降低, 因此, 反义封闭ATM对于白血病原代细胞基因治疗的适应性有待商榷。Objective To study the ataxia telangiectasis mutant (ATM) mRNA expression level in the myeloid leukemia primary cells and tumor cell lines in order to determine the feasibility of antisense blocking the ATM gene therapy to the leukemia.Methods The ATM expression in the normal people and 31 cases of myeloid leukemia and 9 kinds of tumor cell lines was detected by the semi-quantitive RT-PCR.Results The ATM expression was generally low in the leukemia patients and tumor cell lines.Conclusion The adaptability of antisense gene therapy of ATM to the leukemia needs further discussions.

关 键 词：共济失调 毛细血管扩张症 突变基因 白血病 髓性 

分 类 号：R733.7[医药卫生—肿瘤]