固态发酵的参数周期变化及对微生物发酵的影响  

The Periodic Change of Environment Factors in Solid State Fermentation and Effect on Microorganism Fermentation

作　　者：李宏强[1] 陈洪章[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100080

出　　处：《生物工程学报》2005年第3期440-445,共6页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划 (973计划 ) (No .2 0 0 4CB7197OO);中国科学院知识创新工程重要方向性项目 (No .KJCX2_SW_2 0 6);国家高技术研究发展计划 (863计划 ) (No .2 0 0 3AA5 14 0 2 3 )项目资助~~

摘　　要：研究了压力脉动固态发酵反应器内环境参数的周期性变化以及这些周期性的环境刺激对于固态培养的斜卧青霉发酵的影响。研究结果显示:在这种反应器中,在空气压力脉动的带动下,反应器内的温度和空气湿度也会发生较大幅度的周期性变化,变化的周期和空气压力脉动的周期相同,变化的幅度随着压力脉动的幅度增大而增加;在外界周期刺激的条件下,较未加外界周期刺激斜卧青霉的生物量增加了10 4 % ,二氧化碳的产生总量增加了2 2 9%和纤维素酶的产量增加了32 0 % ,数据显示外界周期刺激不仅增加了菌体的生物量同时增加了其代谢活性。The periodic change of environment factors in air pressure oscillation solid state fermentation bioreactor was studied. Based this research the effect of the periodic environment stimulations on Penicillium decumbens JUA10 cultured in solid state substrate was researched too. The research results showed that in this bioreactor air temperature and relative humidity had large amplitude periodic change drived by air pressure oscillation. The changes had same frequency with the air pressure Oscillation and had amplitude that increased with the air pressure increased. When the press lower limit and upper limit were 0.0MPa and 0.2MPa respectively, pulsating period was 20min, contrast to the period gather breath solid state fermentation, the air press pulsating SSF had a 2.29 times total CO\-2 production increase, 3.2 times enzyme activity increase and 1.04 times biomass increase. The data suggested air press pulsating stimulation not only increased biomass but also the metabolic activity.

关 键 词：微生物发酵 周期变化 固态发酵反应器 周期性变化 压力脉动 斜卧青霉 内环境参数 固态培养 环境刺激 研究结果 空气湿度 纤维素酶 二氧化碳 代谢活性 数据显示 生物量 大幅度 外界 

分 类 号：TQ920[轻工技术与工程—发酵工程]