脱氧雪腐镰刀菌烯醇抑制体外培养人外周血单个核细胞低分子量蛋白酶体-2表达  

The Effects of Deoxynivalenol on LMP-2 Expression of Human Peripheral Blood Mononuclear Cells in Vitro

作　　者：李月红[1] 张祥宏[1] 邢凌霄[1] 左连富[2] 严霞[1] 王俊灵[1] 王凤荣[1] 

机构地区：[1]河北医科大学基础医学研究所实验病理室,石家庄市050017 [2]河北省肿瘤研究所细胞室,石家庄市050011

出　　处：《细胞生物学杂志》2005年第3期347-350,共4页Chinese Journal of Cell Biology

基　　金：河北省自然科学基金资助项目(No.301350);科技部重大基础研究项目前期研究专项(No.2001CCC00500)~~~

摘　　要：探讨脱氧雪腐镰刀菌烯醇(DON)对人外周血单个核细胞参与抗原呈递的低分子量蛋白酶体-2(LMP-2)表达的影响。采用流式细胞术(FCM)和半定量RT-PCR方法从蛋白质和mRNA水平分析了不同剂量DON对体外培养人外周血单个核细胞LMP-2分子表达的影响及其量效关系。FCM定量检测结果表明,不同浓度DON处理均可一定程度抑制人外周血单个核细胞LMP-2的表达,50ng/mlDON组、100ng/mlDON组、1000ng/mlDON组和2000ng/mlDON组LMP-2平均荧光强度分别为6.99±0.72、6.21±0.55、5.34±0.56和5.03±0.43,在50～2000ng/mL范围内随着DON浓度增加,外周血单个核细胞LMP-2表达降低,与DON浓度呈显著负相关(r=0.824,P<0.01)。半定量RT-PCR结果显示,不同浓度DON处理均可抑制人外周血单个核细胞LMP-2mRNA表达。DON在蛋白质和mRNA水平可剂量依赖地抑制体外培养的人外周血单个核细胞LMP-2的表达。To explore the effects of deoxynivalenol (DON) on low molecular weight polypeptide-2 (LMP- 2) expression of human peripheral blood mononuclear cells. Effects of deoxynivalenol at different concentration on LMP-2 expression of human peripheral blood mononuclear cells in vitro were studied with flow cytometry (FCM) analysis and RT-PCR. FCM analysis indicated that LMP-2 expression of human peripheral blood mononuclear cells were reduced at protein level in DON treatment groups (50 ng/ml, 100 ng/ml, 1000 ng/ml and 2000 ng/ml), the mean fluorescence intensity of LMP-2 expression in DON groups were 6.99±0.72、6.21±0.55、5.34±0.56 and 5.03±0.43, respectively. As the DON concentration increase from 50 ng/mL to 2000 ng/mL, the expression of LMP-2 was decreased correspondingly and a significant dose-depended response negative correlation could be found between expressions of LMP-2 and DON concentration. RT-PCR also confirmed that DON at higher concen- tration (1000 ng/ml and 2000 ng/ml) could distinct inhibit LMP-2 mRNA expressions. The results suggest that DON could reduce LMP-2 expressions of human peripheral blood mononuclear cells at protein and mRNA level in a concentration-depended manner in vitro.

关 键 词：脱氧雪腐镰刀菌烯醇 体外培养 人外周单个核细胞 低分子量蛋白酶体-2 免疫功能 基因表达 肿瘤 呕吐毒素 粮食污染 

分 类 号：R73-3[医药卫生—肿瘤]