食品中单核增生性李斯特菌的PCR快速检测研究  

Rapid detection of Listeria monocytogenes in food by PCR

作　　者：冯家望[1] 吴小伦[1] 黄云君[1] 王小玉[1] 薄清如[1] 陈静静[1] 

机构地区：[1]珠海出入境检验检疫局,广东珠海519015

出　　处：《中国食品卫生杂志》2005年第3期234-237,共4页Chinese Journal of Food Hygiene

基　　金：国家质量监督检验检疫总局科研项目(2002IK002-08)~~

摘　　要：为提高食品中单增李斯特菌的检测水平,针对单增李斯特菌中多个稳定的特异性基因hlyA、plcB、prfA、iap,设计并筛选出7对引物组成多重-巢式PCR联合检测体系,并结合高灵敏性的聚丙烯酰胺凝胶电泳,对单增李斯特菌进行快速检测,多重PCR的灵敏度达到1×102CFU ml,巢式PCR的灵敏度达到1×10CFU ml。结果表明该检测体系具有快速可靠、灵敏准确及特异性好的特点,而且有效缩短了检验周期,从传统的7～14d缩短到1～2d。According to the Listeria monocytogenes stable specific genes(hlyA,plcB,prfA and iap),the authors desigened and screened out seven primer pairs to compose the multiplex-nested PCR combined detection system,and combined highly sensitive polyacetylamide gel eletrophoresis(PAGE) to detect Listeria monocytogenes.Sensitivity of the multiplex-PCR was 1×102CFU/ml,and that of the nested-PCR was 1×10 CFU/ml.The results show that this detection system is rapid, sensitive accurate and highly specific. It can shorten the detection time from 7～14 days to 1～2 days.=

关 键 词：利斯特氏菌 单核细胞增生 食品 聚合酶链反应 

分 类 号：R155.5[医药卫生—营养与食品卫生学]