金针菇子实体多糖成分的初步研究  

Primary Studies on Polysaccharide Compositions from Flammulina velutipes

作　　者：秦小明[1] 余娟[2] 宁恩创[2] 林华娟[1] 

机构地区：[1]湛江海洋大学食品科技学院,湛江524088 [2]广西大学轻工与食品工程学院,南宁530004

出　　处：《食用菌学报》2005年第2期27-31,共5页Acta Edulis Fungi

摘　　要：初步探讨了金针菇子实体多糖成分的分离与纯化。采用热水抽提、三氯乙酸法脱蛋白、乙醇沉淀获得金针菇子实体粗多糖,然后经DEAE Cellulose阴离子交换柱(B2O74-型)进行分离纯化。结果表明,金针菇粗多糖是一个以葡聚糖为主、由数个多糖组分构成的混合多糖。<Abstrcat> Primary isolation and purification of polysaccharide compositions of Flammulina velutipes was studied. Flammulina velutipes crude polysaccharide was extracted by hot water, deprived of proteins using trichloroacetic acid method and precipitated by ethanol, then the crude polysaccharide was isolated and purificated into three fractions——FVCP-A, FVCP-B and FVCP-C by DEAE-Cellulose column chromatography (B_2O_7^(4-)). The result of monosacharide composition analysis suggested that F. velutipes crude polysaccharides should be the mixed polysaccharides mainly containing glucan and other components.

关 键 词：子实体多糖 金针菇 成分 阴离子交换柱 分离与纯化 热水抽提 氯乙酸法 乙醇沉淀 分离纯化 DEAE 多糖组分 粗多糖 脱蛋白 葡聚糖 

分 类 号：S646.15[农业科学—蔬菜学] TQ281[农业科学—园艺学]