里氏木霉306产t-PA固态发酵条件的研究  

Solid-state fermentation conditions for t-PA production by Trichoderma reesei 306

作　　者：冮洁 杜连祥[1] 路福平[1] 廖海印[2] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222 [2]齐齐哈尔大学生命科学与工程学院,齐齐哈尔161006

出　　处：《工业微生物》2005年第2期14-18,共5页Industrial Microbiology

基　　金：黑龙江省自然科学基金资助项目(No.D0352)

摘　　要：对基因工程菌株里氏木霉(Trichodermareesei)306生物合成组织型纤溶酶原激活剂(t-PA)的固态发酵培养基成分和发酵条件进行了研究;分析了发酵过程中t-PA的生成、总糖的消耗和菌体生长的规律。在优化的固体发酵条件下,t-PA的最大酶活是924.63IU/g干重培养基,较初始条件下提高了5倍。通过浅盘进行了放大实验,最大酶活为983.64IU/g干重培养基,t-PA合成速率为:13.66IU/g干重培养基.h,较三角瓶固态发酵最高产酶提高了6.38%,产酶速率提高了24.07%。Solid-state fermentation conditions of recombinant Trichoderma reesei 306 for t-PA production were investigated. The optimum medium and the optimum fermentation conditions were determined .Under optimized conditions ,the maximum t-PA productivity was enhanced by 5 times than that under the original fermentation conditions.The optimized technology was scaled up in the tray cultivation and the maximum t-PA productivity was 983.64IU/g dry medium, the yield was 13.66 IU/g dry mediumh, increased by 6.38% and 24.07% compared with those of flask cultivation, respectively.

关 键 词：里氏木霉306 组织型纤溶酶原激活剂 T-PA 固态发酵 发酵条件 基因工程药物 

分 类 号：TQ925[轻工技术与工程—发酵工程]