金黄色葡萄球菌肠毒素基因的分型和分布  

Typing and distribution of toxin genes among Staphylococcus aureus isolates from samples of raw milk

作　　者：张严峻[1] 张俊彦[1] 梅玲玲[1] 王志刚[1] 朱敏[1] 王赞信[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310009

出　　处：《中国卫生检验杂志》2005年第6期682-684,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立金黄色葡萄球菌肠毒素基因的检测方法。初步研究生牛奶中金黄色葡萄球菌肠毒素基因的分布状况。方法:应用PCR扩增经分离鉴定的金黄色葡萄球菌肠毒素基因SEA-SEJ,电泳检测扩增结果。结果:建立了金黄色葡萄球菌肠毒素基因PCR检测方法。从生牛奶中分离的29株金黄色葡萄球菌检测到SEA-SED和SEG-SEJ基因,没有检测到SEE基因。毒素基因携带率为65.5%,同时携带2种及以上毒素基因的菌株占37.9%。有SEA基因的占51.7%,含其它传统的毒素基因类型SEB、SEC、SED和SEE基因的菌株只占17.2%,携带新发现的毒素基因SEG、SEH、SEI和SEJ的菌株占41.4%。结论:建立的PCR检测金黄色葡萄球菌的肠毒素基因的方法可用于金黄色葡萄球菌肠毒素基因分型和分布的研究。研究表明,生牛奶中分离的金黄色葡萄球菌带毒率高,而且携带新发现的肠毒素基因的较多。Objective:To develop a typing method for Staphylococcal toxin genes, and to research on the distribution of toxin genes Staphylococcus aureus isolates from raw milk of cows.Methods:Toxin genes were amplified by PCR, and detected by electrophoresis.Results:The typing method for Staphylococccal toxin genes with PCR was developed.The toxin genes of SEA-SED and SEG-SEJ but not SEE could be detected from 29 Staphylococcus aureus isolates from raw milk of cows.Staphylococcus aureus isolates with toxin genes accounted for 65.5% of all the Staphylococcus aureus isolates, the isolates with more than one toxin genes accounted for 37.9%, the isolates with SEA gene accounted for 51.7%, the isolates with other traditional toxin genes only accounted for 17.2%, but the isolates with newly found toxin genes accounted for 41.4%.Conclusion:The developed method for typing Staphylococccal toxin genes with PCR was usableness.Staphylococcus aureus isolates with toxin genes accounted for high ratio, and with newly found toxin genes also accounted for high ratio.

关 键 词：肠毒素基因 金黄色葡萄球菌肠毒素 PCR检测方法 PCR扩增 基因携带率 SEA基因 生牛奶 分布状况 分离鉴定 电泳检测 基因类型 基因分型 E基因 步研究 菌株 SEB SEC SED SEG 带毒率 新发 

分 类 号：R155.5[医药卫生—营养与食品卫生学] R155.31[医药卫生—公共卫生与预防医学]