不同pH提取液对羔羊皱胃酶提取活性的影响  

Influences of different pH buffers on the extracting activity of kid rennet

作　　者：张富新[1] 安森亚 崔馨[3] 

机构地区：[1]陕西师范大学食品工程系,陕西西安710062 [2]郑洲牧业高等专科学校食品系,河南郑州450008 [3]北京科技大学应用科学学院,北京100083

出　　处：《西北农林科技大学学报（自然科学版）》2005年第6期53-56,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基金项目(2003Ci34)

摘　　要：　以关中奶山羊羔羊皱胃为原料,分别用pH4.5,5.4和6.0提取液提取羔羊皱胃酶,并用L9(34)正交试验研究了不同pH提取液对皱胃酶提取效果的影响。结果表明,pH5.4提取液的提取活性最高,pH6.0提取液次之,pH4.5提取液最低;其最佳技术参数为:提取液pH5.4,NaCl质量浓度80g/L,提取温度30℃,皱胃质量与提取液体积之比为1∶20;提取时间48h。<Abstrcat>Using goat kid abomasum of Guanzhong dairy goats as material,kid rennet was extracted with pH 4.5,pH 5.4 and pH 6.0 buffers respectively,and effect of different factors on the extracting activity of kid rennet in different pH buffers was studied by L_9(3~4) orthogonal experiment.The results showed that the extracting activity of kid rennet in pH 5.4 buffer ranked the highest,pH6.0 buffer the second,pH 4.5 buffer the last,and the best technological parameters ware extracting solution pH 5.4,NaCl concentration 80 g/L,extracting temperature 30 ℃,ratio of abomasums and buffer 1∶20,and extracting time 48 hours.

关 键 词：pH提取液 羔羊皱胃酶 酶活性 

分 类 号：S872[农业科学—畜牧兽医] TS201.25[轻工技术与工程—食品科学]