RhBMP-2与D、L-乳酸复合物修复兔下颌骨缺损的实验研究  

Using of PDLLA Combined with BMP to Reconstruct Rabbits' Mandibular Defects

作　　者：童昕[1] 胡勤刚[1] 寿卫东[1] 沈健[2] 陈强[2] 

机构地区：[1]南京大学医学院附属口腔医院口腔颌面外科,江苏南京210008 [2]南京大学化学化工学院,江苏南京210000

出　　处：《口腔颌面外科杂志》2005年第2期141-144,共4页Journal of Oral and Maxillofacial Surgery

基　　金：江苏省卫生厅医药卫生课题资助(H9739)

摘　　要：目的:将聚D、L鄄乳酸(PDLLA)为载体的RhBMP(骨形成蛋白)鄄2缓释系统用于下颌骨缺损的修复,评价其降解缓释特性和诱导成骨作用。方法:低温下制备RhBMP鄄2缓释系统,并将其用于兔下颌骨缺损动物模型的骨缺损修复,设单纯载体材料置入和空白对照组。分别于第2、4、8、12及24周处死动物,取下标本作大体观察、X线片、常规组织学检查和四环素双标记硬组织切片观察,采用Freemax图象分析处理软件计算骨矿化沉积速度。结果:早期在植入物周围组织均存在非特异性炎症反应;4周时炎症反应已明显减轻。4、8、12周时试验组的骨矿化沉积速度分别为1.88、1.4、1.2μm/d,明显优于单纯载体组(P<0.05),但是PDLLA组也存在一定的成骨活性。结论:经物理方法复合的BMP/PDLLA植入物具有良好的生物相容性和诱导成骨能力,其修复颌骨缺损具有较好的效果和临床应用前景。Objective: To evaluate the feasibility and the tissue compatibility of PDLLA combined with BMP in the reconstruction of rabbits' mandibular defects, and to provide a warranty for its clinical application. Methods: PDLLA combined with BMP were implanted in the mandibular defects of rabbits with the control groups of single PDLLA carrier and blank?2, 4, 8 12 and 24 weeks after implantation, the rabbits were killed. The mandibles were examined by X-ray, undecalcified Teteracycline label slide and HE histological observation. The results were analyzed by χ2-test. Results: At the terminal of 2 weeks, non-specific inflammation was found in the tissue around the implants. After 4 weeks, the inflammation response abated obviously. Mineral appositing rate of experimental group in 4, 8, 12 weeks were 1.88 μm/d?1.4 μm/d?1.2 μm/d respectively higher than that of control group(P<0.05). The capacity of inducing osteogenesis in experiment group was clearly superior to control side. But the control group implants also present osteogenesis activity in early stage. Conclusion: The system we used in this study could get good bone reconstruction.

关 键 词：RHBMP-2 下颌骨缺损 实验研究 乳酸 复合物 常规组织学检查 PDLLA 诱导成骨作用 诱导成骨能力 临床应用前景 兔 缓释系统 炎症反应 骨形成蛋白 骨缺损修复 生物相容性 载体材料 动物模型 组织切片 分析处理 非特异性 周围组织 

分 类 号：R782.2[医药卫生—口腔医学]