外显子周期三行为特征的研究  

Study on Period-3 Behavior of Exons

作　　者：田元新[1] 陈超[2] 邹小勇[1] 邱建丁[2] 蔡沛祥[1] 莫金垣[1] 

机构地区：[1]中山大学化学与化学工程学院,广州510275 [2]萍乡高等专科学校化工系,萍乡337055

出　　处：《化学学报》2005年第13期1215-1219,i003,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20475068);广东省自然科学基金(No.031577);湖南大学化学生物传感与计量学国家重点实验室开放基金(No.2003-06)资助项目

摘　　要：基因中蛋白质的编码区具有周期三行为这一规律已成为目前基因预测的理论基础,采用功率谱对外显子的周期三行为特征进行了研究,结果表明:大多数外显子独立存在于基因中时并不具有周期三行为,而当基因被剪切后外显子连在一起编码蛋白质的时候才具有周期三行为.并且这种行为特征与外显子的长度、碱基在密码子三个位置上的分布以及氨基酸密码子的使用偏好均有密切关系,同时符合蛋白质翻译次序的外显子也具有对密码子使用的偏好性,这一研究结果对于提高基因预测的准确率以及内含子功能的研究具有重要意义.It is well-known that base sequences in the protein-coding regions of DNA molecules exhibit a period-3 behavior, which is the theoretical basis of gene prediction. In this work, period-3 behavior of the exons was studied by power spectrum, and the results showed that many exons had no period-3 behavior. After these exons in gene were connected to code proteins, the long coding-protein sequences then displayed the period-3 behavior. The period-3 behavior is related to the distribution of bases and the synonymous codons usage. Each exon was adjusted to make it accord with the translation order, and it has been found that the synonymous codon usage preference is another character of exons. These conclusions would be significant to gene prediction and the study on introns.

关 键 词：外显子 周期三行为特征 基因预测 内含子 功率谱 蛋白质 编码区 

分 类 号：Q75[生物学—分子生物学]