L-亮氨酸产生菌TK0303的5 L罐分批发酵动力学研究  

Study on Batch Fermentation Kinetics of L-Leucine Producing Strain TK0303 in 5-Liter Fermentor

作　　者：宋超先[1] 张文生[1] 谢玉锋[2] 陈宁[3] 张克旭[3] 

机构地区：[1]天津职业大学生物与环境工程学院,天津300402 [2]哈尔滨学院生命科学与化学学院,黑龙江哈尔滨150086 [3]天津科技大学食品科学与生物工程学院,天津300222

出　　处：《化学与生物工程》2005年第7期27-29,共3页Chemistry & Bioengineering

摘　　要：对L-亮氨酸产生菌黄色短杆菌TK0303(Met-+2-TAr+-αABr+β-HLr+SGr+Rifr800μg.mL-1)进行了5L发酵罐分批发酵试验,该菌株发酵68h,产L-亮氨酸26.44g.L-1。以5L罐分批发酵试验数据为依据,对L-亮氨酸分批发酵动力学进行了研究,建立了菌体生长、产物形成和基质消耗的动力学模型,并应用MATALAB软件进行模型拟合,拟合模型较好地反映了L-亮氨酸分批发酵过程。The batch fermentation was performed with an L-leucine producing strain, Brevibacterium flavum TK0303(Met^(-)+2-TA^(r)+α-AB^(r)+β-HL^(r)+SG^(r)+Rif^(r) (800 μgmL^(-1)))in 5-liter fermentor. The result showed that the strain could produce L-leucine (26.44 gL^(-1)) after fermentation for 68 hours. The L-leucine batch fermentation kinetics based on the experimental data from the batch fermentation in 5-liter fermentor was studied. Three kinetic models were constructed which could reflect the regularity of growth, product formation and substrate consumption in the process of batch fermentation. Then, the better models were obtained through proper analysis by software MATALAB.

关 键 词：L-亮氨酸 分批发酵 动力学 MATALAB软件 

分 类 号：TQ922[轻工技术与工程—发酵工程]