草甘膦诱导表达的大豆与棉花EST序列的分离  

Isolation of Glyphosate-induced EST Sequences of Soybean and Cotton

作　　者：尉万聪[1] 张锐[1] 郭三堆[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《中国生物工程杂志》2005年第7期80-85,共6页China Biotechnology

基　　金：国家"863"计划资助项目(2001AA222101)

摘　　要：利用差异显示PCR方法分离了63个草甘膦诱导后在大豆和棉花中差异表达的片段,测序分析结果表明属于33个草甘膦诱导的大豆和棉花EST序列。通过在GenBank中进一步比时研究发现:约85%的EST序列与水杨酸、冷、创伤、氧化等非生物胁迫诱导后表达库中的EST序列有高达95%以上的同源性,由此可推测这些基因参与了植物对非生物胁迫的反应过程。草甘膦诱导后高表达EST、序列的获得将有利于进一步分离相关非生物胁迫诱导表达基因及启动子,研究其转录调控的机理,有望建立草甘膦诱导系统。从而解决组成型表达造成外源基因在植物体所有发育阶段和所有组织部位表达,造成植物体能量浪费。63 ESTs sequences were isolated by differential display PCR from soybean and cotton seedlings. Web based alignment showed that these ESTs sequences belongs to 33 genes, and they are all about 95% homologous to abiotic factor induced genes. The results indicated that these genes involved in abiotic response process. Isolation of glyphosate-induced higher expression EST sequence will be benefit to isolate the gene and promoter, investigate the mechanism of regulation, and construct a glyphosate-inducible system. Use this system, the gene expression can be drived only when needed, and reduce the plant energy waste.

关 键 词：EST序列 草甘膦 分离 棉花 大豆 诱导表达 非生物胁迫 GENBANK PCR方法 组成型表达 差异表达 差异显示 分析结果 反应过程 表达基因 转录调控 诱导系统 发育阶段 外源基因 植物体 水杨酸 同源性 启动子 高表达 测序 

分 类 号：Q78[生物学—分子生物学] TQ457.29[化学工程—农药化工]