超声波平板法检测金黄色葡萄球菌生物被膜的研究  

STUDY ON THE DETECTION OFSTAPHYLOCOCCUS AUREUSBIOFILM BY ULTRASONIC-PLATE METHOD

作　　者：黄静[1] 韩北忠[1] 李春雷[2] 彭坚[1] 尹利端[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京出入境检验检疫局,北京100022

出　　处：《河南工业大学学报（自然科学版）》2005年第3期22-24,29,共4页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家质量检验检疫总局科研项目(20031K052)

摘　　要：以金黄色葡萄球菌(StaphylococcusaureusAS1.89)形成的生物被膜为研究对象,采用扫描电镜(SEM)观察生物被膜的形态结构;对棉花擦拭法和超声波平板法用于生物被膜的活菌计数进行了比较;最后测定了超声波作用不同时间去除生物被膜的量.结果表明:超声波平板法对生物被膜的活菌计数优于棉花擦拭法;超声波作用2min能去除大部分生物被膜.The Biofilm morphological structure ofStaphylococcus aureus(AS 1.89) was observed with scanning elec- tronic microscope (SEM). The plate-swabbing method was compared with ultrasonic-plate method when they were used to detect the viable counts of bacteria in biofilm. At last, biofilm amount removed by ultrasonic-plate method with different ultrasonic treating time was measured. The result showed that the biofilm amount removed by ultrason- ic-plate method was higher than that removed by plate-swabbing method. After being treated by ultrasonic for 2 min, most biofilm on the surface was removed·

关 键 词：生物被膜 超声波平板法 扫描电镜 银染法 

分 类 号：TS201.3[轻工技术与工程—食品科学]