对硫磷快速ELISA检测方法的建立  

Modified ELISA for quantitation of parathion in agricultural products

作　　者：芮玉奎[1] 王茜[1] 黄昆仑[1] 郭晶[1] 罗云波[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,农业部农产品质量监督检验测试中心,北京100083

出　　处：《中国食品卫生杂志》2005年第4期315-317,共3页Chinese Journal of Food Hygiene

摘　　要：由于对硫磷的残留问题及其危害,迫切需要建立一种快速有效的农药检测方法。将对硫磷苯环上的硝基还原为氨基,得到了具有氨基活性的半抗原化合物———氨基对硫磷,然后采用重氮法将氨基-对硫磷与牛血清白蛋白(BSA)或卵清白蛋白(OVA)相偶联,分别合成了免疫原和包被抗原:氨基对硫磷-BSA、氨基对硫磷-OVA。对合成的抗原进行紫外可见扫描,确证偶联成功。用合成的免疫抗原免疫实验用大白兔,制备对硫磷的多克隆抗血清,效价达到1∶1×106以上。对硫磷抗体除了与甲基对硫磷具有极其微弱的交叉反应以外,与甲胺磷和毒死蜱交叉反应低于0.1%,最低检测限2.0ngg,回收率为93.3%。该方法简便、快速、灵敏,适用性强。A quick and effective method to dispose the problem of excessive pesticide residues in agricultural products was reported. Parathion was conjugated by diazotization with OVA and BSA to prepare connective antigen (OVA- parathion) and immune antigen (BSA- parathion). They were then identified by ultraviolet radiation scanning and electrophoresis, and the immune antigen was injected into New Zealand rabbit to obtain the polyclonal antibody, whose efficiency value was 1∶1×10~6. The crossover reaction of the antibody against methyl- parathion was 0.98%, and against methamidophos and chlorpyrifos was <0.1%. The detection limit was 2.0 ng/g. This method have comparable to (even exceeded) the method using the test kit.

关 键 词：检测方法 ELISA 牛血清白蛋白 交叉反应 卵清白蛋白 甲基对硫磷 残留问题 硝基还原 包被抗原 紫外可见 免疫实验 免疫抗原 氨基 化合物 半抗原 免疫原 合成 大白兔 抗血清 毒死蜱 甲胺磷 检测限 回收率 适用性 偶联 农药 

分 类 号：S481.8[农业科学—农药学]