苹果汁中棒曲霉素主要产生菌的ITS序列分析鉴定  

Identification of dominating patulin-producing strain by ITS-DNA sequence analysis in apple juice concentrates

作　　者：苏青峰[1] 岳田利[1] 袁亚红[1] 高振鹏[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第7期131-134,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十五"科技攻关项目(2001BA51A15);科技部西部专项(2001BA901A19)

摘　　要：从浓缩苹果汁生产原料中分离出多种棒曲霉素产生菌,形态学初步鉴定表明其均为青霉属和曲霉属真菌。为确定其分类地位,又用ITS-DNA测序鉴定进行了验证。将ITS-rDNA提取后进行PCR扩增,测序后提交到EuropeanMolecularBiologyLaboratory(EMBL)核酸数据库,通过Blast程序,将ITS-rDNA序列与Genebank中的核酸序列进行对照,结果确定其确为青霉属与曲霉属菌株。Several patulin-producing strains isolated from raw material of apple juice concentrates were identificated in morphology,in which all of them were penicillium and aspergillus.Then ITS-rDNA sequence analysis was used to confirm it.The ITS-rDNA sequence was submitted to european molecular biology laboratory (EMBL),and contrasted with DNA sequence in genebank and confirmed that they were penicillium and aspergillus.

关 键 词：棒曲霉素 扩张青霉 ITS-RDNA PCR 浓缩苹果汁 

分 类 号：Q93-331[生物学—微生物学] TS201.3[轻工技术与工程—食品科学]